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S100P Recombinant Rabbit Monoclonal Antibody [JF95-08]

Usd: 385

Specification

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Catalog# ET1702-82

S100P Recombinant Rabbit Monoclonal Antibody [JF95-08]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

S100P Recombinant Rabbit Monoclonal Antibody [JF95-08]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human S100P aa 51-95 / 95.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 10 kDa

Positive Control

Hep G2 (Human hepatocellular carcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, human spleen tissue, human stomach tissue.

Conjugation

unconjugated

Clone Number

JF95-08

RRID

AB_3070342

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200

  • IP

  • 1:50-1:100

Target

Function

S-100 proteins are small dimeric members of the EF-Hand superfamily that participate in moderating intracellular calcium signals by binding to and regulating specific proteins in a calcium-dependent manner. S-100P is a survival factor that is associated with different types of tumors and can bind and regulate effector proteins. R1881, a synthetic androgen, regulates S-100P expression. S-100P interacts with a receptor for advanced glycation end products (RAGE) and activates it, thereby increasing the rates of cell growth, division, migration and invasion. This suggests that S-100P acts in an auto-crine manner through RAGE to trigger cell proliferation and survival. S-100P may also positively affect anchorage-independent growth to improve tumor formation. S-100P monomers strongly interact with one another, but not with other S-100 polypeptides, suggesting that homodimer formation is necessary for S-100P to function. The S-100P dimers are then stabilized by hydrophobic contacts.

Background References

1. Shen Z.Y., et al. 2016. Analysis of the predictive efficiency of S100P on adverse prognosis and the pathogenesis of S100P-mediated invasion and metastasis of colon adenocarcinoma. Cancer Genet 209:143-153.

2. Li Z., et al. 2016. LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway. Cancer Lett. 372:239-250.

Sequence Similarity

Belongs to the S-100 family.

Tissue Specificity

Detected in all of the tissues except brain, testis and small intestine, expression level is higher in placenta, heart, lung, skeletal muscle, spleen and leukocyte. Up-regulated in various pancreatic ductal adenocarcinomas and pancreatic intraepithelial neoplasias.

Subcellular Location

Nucleus, Cytoplasm, Cell projection.

UNIPROT

SwissProt: P25815 Human

Synonyms

MIG9 antibody

Migration inducing gene 9 antibody

Protein S100-E antibody

Protein S100-P antibody

Protein S100P antibody

S100 calcium binding protein P antibody

S100 calcium-binding protein P antibody

S100 P antibody

S100E antibody

S100P antibody

Expand

Images

  • Western blot analysis of S100P on different lysates with Rabbit anti-S100P antibody (<a href="/products/ET1702-82" style="font-weight: bold;text-decoration: underline;">ET1702-82</a>) at 1/1,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate<br />Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1702-82" style="font-weight: bold;text-decoration: underline;">ET1702-82</a>, 1/,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 10 kDa<br />Observed band size: 10 kDa

    Western blot analysis of S100P on different lysates with Rabbit anti-S100P antibody (ET1702-82) at 1/1,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
    Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1702-82, 1/,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 10 kDa
    Observed band size: 10 kDa

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-82" style="font-weight: bold;text-decoration: underline;">ET1702-82</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1702-82" style="font-weight: bold;text-decoration: underline;">ET1702-82</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-S100P antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-82, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"