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HRP Conjugated GFP Recombinant Rabbit Monoclonal Antibody [JF51-05]

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Specification

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Catalog# ET1702-69

HRP Conjugated GFP Recombinant Rabbit Monoclonal Antibody [JF51-05]

Application

  • WB

Reactivity

  • Species independent

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated GFP Recombinant Rabbit Monoclonal Antibody [JF51-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant full length protein of Aequorea victoria GFP.

Species Reactivity

Species independent

Validated Applications

WB

Positive Control

293T transfected with GFP cell lysates, K-562 transfected with GFP cell lysates.

Conjugation

HRP

Clone Number

JF51-05

RRID

AB_3070329

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000-1:40,000

Target

Function

The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.

Background References

1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.

2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.

UNIPROT

SwissProt: P42212 Aequorea victoria

Synonyms

GFP antibody

Green fluorescent protein antibody

yfp antibody

Images

  • Western blot analysis of GFP on 293T transfected with GFP cell lysates with Rabbit anti-GFP antibody (<a href="/products/ET1702-69" style="font-weight: bold;text-decoration: underline;">ET1702-69</a>) at 1/40,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 4 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-69" style="font-weight: bold;text-decoration: underline;">ET1702-69</a>) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GFP on 293T transfected with GFP cell lysates with Rabbit anti-GFP antibody (ET1702-69) at 1/40,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 4 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-69) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Western blot analysis of GFP on K-562 transfected with GFP cell lysates with Rabbit anti-GFP antibody (<a href="/products/ET1702-69" style="font-weight: bold;text-decoration: underline;">ET1702-69</a>) at 1/10,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Exposure time: 30 seconds;<br /><br />120% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-69" style="font-weight: bold;text-decoration: underline;">ET1702-69</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GFP on K-562 transfected with GFP cell lysates with Rabbit anti-GFP antibody (ET1702-69) at 1/10,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Exposure time: 30 seconds;

    120% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-69) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation