FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017]
Catalog# ET1702-44
FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
FOXP1 Recombinant Rabbit Monoclonal Antibody [JF1017]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human FOXP1 aa 615-653 / 677.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
77/75 kDa
Positive Control
Hela cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human colon carcinoma tissue, human breast carcinoma tissue, MCF-7.
Conjugation
unconjugated
Clone Number
JF1017
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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FC
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. The gene encoding human FOXP1 maps to chromosome 3p14.1. The gene encoding human FOXP2 maps to chromosome 7q31. The gene encoding FOXP3, a third member of this family, maps to chromosome Xp11.23-Xq13.3. Mutations in this gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein, also known as scurfin, is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T-cell activation.
Background References
1. Hua ZL et al. Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems. Neural Dev 10:21 (2015).
2. Lu TX et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015).
Tissue Specificity
Isoform 8 is specifically expressed in embryonic stem cells.
Subcellular Location
Nucleus.
Synonyms
12CC4 antibody
FLJ23741 antibody
Fork head related protein like B antibody
Forkhead box P1 antibody
Forkhead box protein P1 antibody
FOX P1 antibody
FOXP 1 antibody
foxp1 antibody
FOXP1_HUMAN antibody
Glutamine rich factor 1 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-FOXP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-FOXP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1702-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of FOXP1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1702-44, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Western blot analysis of FOXP1 on Jurkat cell lysates with Rabbit anti-FOXP1 antibody (ET1702-44) at 1/5,000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 1 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1702-44, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 75.3 kDa
Observed band size: 75/77 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"