mGluR2 Recombinant Rabbit Monoclonal Antibody [JJ091-09]
Catalog# ET1701-74
mGluR2 Recombinant Rabbit Monoclonal Antibody [JJ091-09]
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
mGluR2 Recombinant Rabbit Monoclonal Antibody [JJ091-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human mGluR2 aa 750-872/872.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IF-Tissue, IHC-P
Target Molecular Weight
Predicted band size: 96 kDa
Positive Control
SH-SY-5Y, Hela, SW480, mouse brain tissue, mouse brain tissue lysates, rat brain tissue.
Conjugation
unconjugated
Clone Number
JJ091-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
The mGluR proteins (metabotropic glutamate receptors) are members of the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic glutamate receptors). The eight currently known mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G-protein that inhibits adenylate cyclase activity. mGluR-2, which may interact with GRASP, acts as a receptor for glutamate. It may also be involved in the regulation of neurotransmission suppression and in synaptogenesis or synaptic stablization.
Background References
1. Kurita M et al. Repressive epigenetic changes at the mGlu2 promoter in frontal cortex of 5-HT2A knockout mice. Mol Pharmacol 83:1166-75 (2013).
2. Konermann S et al. Optical control of mammalian endogenous transcription and epigenetic states. Nature 500:472-6 (2013).
Sequence Similarity
Belongs to the G-protein coupled receptor 3 family.
Tissue Specificity
Detected in brain cortex (at protein level). Widely expressed in different regions of the adult brain as well as in fetal brain.
Subcellular Location
Cell membrane, Cell junction, Cell projection.
Synonyms
AMPA selective glutamate receptor 2 antibody
GLUR2 antibody
GLURB antibody
Glutamate metabotropic receptor 2 antibody
Glutamate receptor homolog antibody
Glutamate receptor metabotropic 2 antibody
GPRC1B antibody
GRM2 antibody
GRM2_HUMAN antibody
Metabotropic glutamate receptor 2 antibody
ExpandImages
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ICC staining mGluR2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining mGluR2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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ICC staining mGluR2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of mGluR2 on mouse brain tissue lysates with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 96 kDa
Observed band size: 110 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-74) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-mGluR2 antibody (ET1701-74) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-74) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"