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SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]

Usd: 385

Specification

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Catalog# ET1701-62

SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SUZ12 Recombinant Rabbit Monoclonal Antibody [JJ09-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SUZ12 aa 1-150/739.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IP

Target Molecular Weight

Predicted band size: 83 kDa

Positive Control

MCF-7 cells lysates, NIH/3T3 cell lysate, PC-12 cell lysate, Raw264.7 cell lysate, SW480, MCF-7, Hela, SKOV-3.

Conjugation

unconjugated

Clone Number

JJ09-04

RRID

AB_3070226

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

Target

Function

The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis. The mammalian PcG proteins are regulatory proteins important for Hox gene expression, axial skeleton development and the control of proliferation and survival of hematopoietic cells. By inducing changes in chromatin structure, the PcG proteins are part of a cellular memory system that is responsible for gene activity being inherited to progeny cells. PcG proteins silence gene expression through the formation of multimeric protein complexes with different compositions. Manipulating the expression-levels of various PcG proteins in mammalian cell lines results in cellular transformation, which may be a link between the chromatin-associated PcG proteins and cancer. Polycomb protein SUZ12, also designated ChET 9 protein or joined-to-JAZF1 protein, is a nuclear protein belonging to the VEFS (VRN2-EMF2-FIS2-SUZ12) family. SUZ12 has been detected at the breakpoints of a certain recurrent chromosomal translocation which has been reported in endometrial stromal sarcoma. It is a component of the PRC2 complex, composed of EED, EZH2, SUZ12/JJAZ1, RBBP4 and RBBP7.

Background References

1. Heubach J et al. The long noncoding RNA HOTAIR has tissue and cell type-dependent effects on HOX gene expression and phenotype of urothelial cancer cells. Mol Cancer 14:108 (2015).

2. Negishi M et al. A New lncRNA, APTR, Associates with and Represses the CDKN1A/p21 Promoter by Recruiting Polycomb Proteins. PLoS One 9:e95216 (2014).

Sequence Similarity

Belongs to the VEFS (VRN2-EMF2-FIS2-SU(Z)12) family.

Tissue Specificity

Overexpressed in breast and colon cancer.

Post-translational Modification

Sumoylated, probably by PIAS2.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q15022 Human

SwissProt: Q80U70 Mouse

Entrez Gene: 688041 Rat

Synonyms

ChET 9 protein antibody

CHET9 antibody

Chromatin precipitated E2F target 9 protein antibody

JJAZ1 antibody

Joined to JAZF1 protein antibody

KIAA0160 antibody

Polycomb protein SUZ12 antibody

Suppressor of zeste 12 homolog antibody

Suppressor of zeste 12 protein homolog antibody

SUZ12 antibody

Expand

Images

  • IF-cell staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF-cell staining SUZ12 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • IF-cell staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF-cell staining SUZ12 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • IF-cell staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF-cell staining SUZ12 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • IF-cell staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF-cell staining SUZ12 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Western blot analysis of SUZ12 on different lysates with Rabbit anti-SUZ12 antibody (<a href="/products/ET1701-62" style="font-weight: bold;text-decoration: underline;">ET1701-62</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 (Human breast cancer cell) cell lysate<br />Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 1 minutes; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1701-62" style="font-weight: bold;text-decoration: underline;">ET1701-62</a>, 1/1000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 83 kDa<br />Observed band size: 83 kDa

    Western blot analysis of SUZ12 on different lysates with Rabbit anti-SUZ12 antibody (ET1701-62) at 1/1,000 dilution.

    Lane 1: MCF7 (Human breast cancer cell) cell lysate
    Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 1 minutes; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1701-62, 1/1000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"