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AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

Usd: 385

Specification

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Catalog# ET1701-26

AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

AGO3 Recombinant Rabbit Monoclonal Antibody [JJ201-07]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human EIF2C3/ Argonaute 3 aa 689-860/860.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, FC

Molecular Weight

Predicted band size: 97 kDa

Positive Control

HeLa cell lysate, C6 cell lysate, N2A, SHG-44, F9, NCCIT, rat spinal cord tissue, rat brain tissue, mouse brain tissue, rat testis tissue.

Conjugation

unconjugated

Clone Number

JJ201-07

RRID

AB_3070194

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100-1:500

  • IF-Tissue

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Eukaryotic translation initiation factor 2C (eIF2C) proteins (argonaute family) influence RNA interference (RNAi) as components of the RNA-inducible silencing complex (RISC) or microRNA (miRNA)-containing ribonucleoprotein particle (miRNP). Small RNAs, including small interfering RNAs (siRNAs) and miRNAs, can silence target genes through mechanisms that utilize RISC or miRNP particles. eIF2C1 (argonaute 1, AGO1, eIF2C, GERP95, Q99) and Dicer1 play a coordinated role in siRNA-mediated gene silencing. eIF2C2 (Slicer, argonaute 2, AGO2, Q10) is a RISC component that can concentrate in cytoplasmic processing bodies (P-bodies) and catalyze mRNA cleavage. Mammalian P-bodies contain mRNAs and have an association with miRNA-induced translational silencing and siRNA-induced mRNA degradation. Additional eIF2C proteins include eIF2C3 (argonaute 3, AGO3), eIF2C4 (argonaute 4, AGO4) and meIF2c5 (mouse argonaute 5).

Background References

1. Hein M.Y., et al. 2015. A human interactome in three quantitative dimensions organized by stoichiometries and abundances. Cell 163:712-723.

2. Schurmann N., et al. 2013. Molecular dissection of human Argonaute proteins by DNA shuffling. Nat. Struct. Mol. Biol. 20:818-826.

Sequence Similarity

Belongs to the argonaute family. Ago subfamily.

Subcellular Location

Cytoplasm,Nucleus.

UNIPROT

SwissProt: Q9H9G7 Human

SwissProt: Q8CJF9 Mouse

Unigene: 92025 Rat

Synonyms

5730550L01Rik antibody

Ago 3 antibody

Ago3 antibody

AGO3_HUMAN antibody

argonaute 3 antibody

Argonaute3 antibody

eIF 2C 3 antibody

eIF-2C 3 antibody

eIF2C 3 antibody

Eif2c3 antibody

Expand

Images

  • Western blot analysis of AGO3 on different lysates with Rabbit anti-AGO3 antibody (<a href="/products/ET1701-26" style="font-weight: bold;text-decoration: underline;">ET1701-26</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 97 kDa<br />Observed band size: 97 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-26" style="font-weight: bold;text-decoration: underline;">ET1701-26</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AGO3 on different lysates with Rabbit anti-AGO3 antibody (ET1701-26) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 97 kDa
    Observed band size: 97 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-26) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining AGO3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining AGO3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining AGO3 in NCCIT cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-AGO3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"