14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ083-6]
Catalog# ET1701-2
14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ083-6]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
14-3-3 Recombinant Rabbit Monoclonal Antibody [JJ083-6]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human 14-3-3 aa 1-126 / 246.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 28 kDa
Positive Control
HEK-293 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse skin tissue.
Conjugation
unconjugated
Clone Number
JJ083-6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IHC-P
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1:50-1:200
Target
Function
14-3-3 proteins are a family of conserved regulatory molecules that are expressed in all eukaryotic cells. 14-3-3 proteins have the ability to bind a multitude of functionally diverse signaling proteins, including kinases, phosphatases, and transmembrane receptors. More than 200 signaling proteins have been reported as 14-3-3 ligands. 14-3-3 proteins play an isoform-specific role in class switch recombination. They are believed to interact with the protein Activation-Induced (Cytidine) Deaminase in mediating class switch recombination. Phosphorylation of Cdc25C by CDS1 and CHEK1 creates a binding site for the 14-3-3 family of phosphoserine binding proteins. Binding of 14-3-3 has little effect on Cdc25C activity, and it is believed that 14-3-3 regulates Cdc25C by sequestering it to the cytoplasm, thereby preventing the interactions with CycB-Cdk1 that are localized to the nucleus at the G2/M transition.
Background References
1. Lehmann LH et al. Essential role of sympathetic endothelin A receptors for adverse cardiac remodeling. Proc Natl Acad Sci U S A 111:13499-504 (2014).
2. Dettmer U et al. In vivo cross-linking reveals principally oligomeric forms of a-synuclein and -synuclein in neurons and non-neural cells. J Biol Chem 288:6371-85 (2013).
Sequence Similarity
Belongs to the 14-3-3 family.
Post-translational Modification
The alpha, brain-specific form differs from the beta form in being phosphorylated. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.
Subcellular Location
Cytoplasm, Melanosome, Vacuole membrane.
UNIPROT
Synonyms
14 3 3 antibody
14 3 3 protein beta antibody
14 3 3 protein beta/alpha antibody
14 3 3 protein zeta antibody
14 3 3 zeta antibody
14-3-3 protein beta/alpha antibody
14-3-3 protein/cytosolic phospholipase A2 antibody
1433B_HUMAN antibody
GW128 antibody
HS1 antibody
ExpandImages
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Western blot analysis of 14-3-3 on different lysates with Rabbit anti-14-3-3 antibody (ET1701-2) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-2, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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