HSPA2 Recombinant Rabbit Monoclonal Antibody [JJ08-77]
Catalog# ET1701-15
HSPA2 Recombinant Rabbit Monoclonal Antibody [JJ08-77]
-
WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
HSPA2 Recombinant Rabbit Monoclonal Antibody [JJ08-77]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human HSPA2 aa 448-639 / 639.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 70 kDa
Positive Control
MCF-7 cell lysate, Hela cell lysate, A431 cell lysate, mouse testis tissue, rat testis tissue, human smooth muscle tissue.
Conjugation
unconjugated
Clone Number
JJ08-77
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000-1:2,000
-
IHC-P
-
1:400-1:1,000
Target
Function
HSPA2(heat shock 70kDa protein 2) also known as HEAT-SHOCK PROTEIN, 70-KD, 2, HSP70-2, HEAT-SHOCK PROTEIN, 70-KD, 3 or HSP70-3. Analysis of the sequence indicated that HSPA2 is the human homolog of the murine Hsp70-2 gene, with 91.7% identity in the nucleotide coding sequence and 98.2% in the corresponding amino acid sequence.HSPA2 has less amino acid homology to the other members of the human HSP70 gene family. HSPA2 is constitutively expressed in most tissues, with very high levels in testis and skeletal muscle. The HSPA2 gene is located on chromosome 14q22-q24. Immunohistochemical analysis detected weak expression of HSPA2 in spermatocytes and stronger expression in spermatids and in the tail of mature sperm. HSPA2 may be critical to sperm maturation through its role as a protein chaperone.
Background References
1. Rual JF, et al. (2005). Towards a proteome-scale map of the human protein-protein interaction network. Nature. 437 (7062): 1173–8.
2. Gerhard DS, et al. (2004). The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Genome Res. 14 (10B): 2121–7.
Sequence Similarity
Belongs to the heat shock protein 70 family.
Subcellular Location
Cytoplasm.
Synonyms
70kDa antibody
Hcp70.2 antibody
Heat shock 70 kDa protein 2 antibody
Heat shock protein 2 antibody
Heat shock protein 70.2 antibody
Heat shock related 70 kDa protein 2 antibody
Heat shock-related 70 kDa protein 2 antibody
Heat-shock protein, 70-KD, 2 antibody
Heat-shock protein, 70-KD, 3 antibody
HSP70 2 antibody
ExpandImages
-
Western blot analysis of HSPA2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-15, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 3: A431 cell lysate -
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-HSPA2 antibody (ET1701-15) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HSPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
-
Immunohistochemical analysis of paraffin-embedded human smooth muscle tissue using anti-HSPA2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-15, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"