Cdc37 Recombinant Rabbit Monoclonal Antibody [JJ087-06]
Catalog# ET1701-14
Cdc37 Recombinant Rabbit Monoclonal Antibody [JJ087-06]
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WB
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Cdc37 Recombinant Rabbit Monoclonal Antibody [JJ087-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cdc37 aa 1-50 / 378.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, FC, IP
Molecular Weight
Predicted band size: 44 kDa
Positive Control
A431 cell lysate, K562 cell lysate, mouse liver tissue, mouse placenta tissue, Hela.
Conjugation
unconjugated
Clone Number
JJ087-06
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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FC
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1:50-1:100
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
Hsp90 co-chaperone Cdc37 is a protein that in humans is encoded by the CDC37 gene. The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Saccharomyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK, as well as eIF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.
Background References
1. Liu, W. et al. 2015. Phosphorylated and unphosphorylated serine 13 of CDC37 stabilize distinct interactions between its client and HSP90 binding domains. Biochemistry. 54: 1493-504.
2. Maruyama, K. et al. 2015. 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival. J. Biol. Chem.. 290: 9377-86.
Sequence Similarity
Belongs to the CDC37 family.
Post-translational Modification
Constitutively sumoylated by UBE2I.
Subcellular Location
Cytoplasm.
Synonyms
CDC 37 antibody
Cdc37 antibody
CDC37 cell division cycle 37 homolog antibody
CDC37 cell division cycle 37 S cerevisiae homolog antibody
CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
Cdc37 homolog antibody
CDC37 protein antibody
CDC37_HUMAN antibody
CDC37A antibody
cell division cycle 37 antibody
ExpandImages
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Western blot analysis of Cdc37 on different lysates with Rabbit anti-Cdc37 antibody (ET1701-14) at 1/500 dilution.
Lane 1: A431 cell lysate
Lane 2: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-14) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Cdc37 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Cdc37 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-14, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of Cdc37 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-14, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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