CDC42EP1 Recombinant Rabbit Monoclonal Antibody [SD08-54]
Catalog# ET1612-95
CDC42EP1 Recombinant Rabbit Monoclonal Antibody [SD08-54]
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WB
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Human
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unconjugated
Overview
Product Name
CDC42EP1 Recombinant Rabbit Monoclonal Antibody [SD08-54]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CDC42EP1 aa 310-359 / 391.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa cell lysate, HUVEC cell lysate, HEK-293 cell lysate, K-562 cell lysate.
Conjugation
unconjugated
Clone Number
SD08-54
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Cdc42 effector protein 1 is a protein that in humans is encoded by the CDC42EP1 gene. CDC42 is a member of the Rho GTPase family that regulates multiple cellular activities, including actin polymerization. The protein encoded by this gene is a CDC42 binding protein that mediates actin cytoskeleton reorganization at the plasma membrane. The encoded protein, which is secreted, is primarily found in bone marrow. Two transcript variants encoding different isoforms have been found for this gene.
Background References
1. Guo A., et al. 2014. Immunoaffinity enrichment and mass spectrometry analysis of protein methylation. Mol. Cell. Proteomics 13:372-387.
2. Bian Y., et al. 2014. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J. Proteomics 96:253-262.
Sequence Similarity
Belongs to the BORG/CEP family.
Tissue Specificity
Endothelial and bone marrow stromal cells.
Subcellular Location
Endomembrane system, Cytoplasm.
UNIPROT
Synonyms
55 kDa bone marrow stromal/endothelial cell protein antibody
Binder of Rho GTPases 5 antibody
Bone marrow stromal/endothelial cell protein, 55-KD antibody
BORG 5 antibody
BORG5 antibody
BORG5_HUMAN antibody
CDC42 effector protein (Rho GTPase binding) 1 antibody
Cdc42 effector protein 1 antibody
Cdc42ep1 antibody
CEP 1 antibody
ExpandImages
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Western blot analysis of CDC42EP1 on different lysates with Rabbit anti-CDC42EP1 antibody (ET1612-95) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HUVEC cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-95) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"