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Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]

Usd: 385

Specification

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Catalog# ET1612-94

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Cdc27 Recombinant Rabbit Monoclonal Antibody [SD85-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Cdc27 aa 662-824 / 824.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 92 kDa

Positive Control

HeLa cell lysate, MCF7 cell lysate, PC-12 cell lysate, Rat thymus tissue lysate, Neuro-2a cell lysate, F9 cell lysate, human colon carcinoma tissue, human prostate tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

SD85-02

RRID

AB_3070171

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200

Target

Function

Cell division cycle protein 27 homolog is a protein that in humans is encoded by the CDC27 gene. The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

Background References

1. Ren YQ et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015).

2. Holt JE et al. Spatial regulation of APCCdh1-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes. Development 137:1297-304 (2010).

Sequence Similarity

Belongs to the APC3/CDC27 family.

Post-translational Modification

Phosphorylated. Phosphorylation on Ser-426 and Thr-446 occurs specifically during mitosis.

Subcellular Location

Nucleus, Cytoplasm, cytoskeleton, spindle.

UNIPROT

SwissProt: P30260 Human

SwissProt: A2A6Q5 Mouse

SwissProt: Q4V8A2 Rat

Synonyms

ANAPC3 antibody

Anaphase Promoting Complex 3 antibody

Anaphase promoting complex protein 3 antibody

Anaphase Promoting Complex Subunit 3 antibody

Anaphase-promoting complex subunit 3 antibody

APC 3 antibody

APC3 antibody

Cdc 27 antibody

Cdc27 antibody

CDC27 homolog antibody

Expand

Images

  • Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate (20 µg/Lane)<br />Lane 2: MCF7 cell lysate (20 µg/Lane)<br />Lane 3: PC-12 cell lysate (20 µg/Lane)<br />Lane 4: Rat thymus tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 20 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate (20 µg/Lane)
    Lane 2: MCF7 cell lysate (20 µg/Lane)
    Lane 3: PC-12 cell lysate (20 µg/Lane)
    Lane 4: Rat thymus tissue lysate (40 µg/Lane)

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 20 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-94) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/5,000 dilution.<br /><br />Lane 1: Neuro-2a cell lysate<br />Lane 2: F9 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 92 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Cdc27 on different lysates with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/5,000 dilution.

    Lane 1: Neuro-2a cell lysate
    Lane 2: F9 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 92 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-94) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cdc27 antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Cdc27 antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cdc27 antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1612-94" style="font-weight: bold;text-decoration: underline;">ET1612-94</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cdc27 antibody (ET1612-94) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-94) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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