CDC23 Recombinant Rabbit Monoclonal Antibody [SD08-60]
Catalog# ET1612-84
CDC23 Recombinant Rabbit Monoclonal Antibody [SD08-60]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
CDC23 Recombinant Rabbit Monoclonal Antibody [SD08-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cdc23 aa 521-570 / 597.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 69 kDa
Positive Control
HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat kidney tissue lysate.
Conjugation
unconjugated
Clone Number
SD08-60
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
Cell division cycle 23 homolog (S. cerevisiae), also known as CDC23, is a protein that, in humans, is encoded by the CDC23 gene. The CDC23 protein shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.
Background References
1. Achari C et al. Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells. BMC Cancer 14:538 (2014).
2. Sako K et al. Emi2 mediates meiotic MII arrest by competitively inhibiting the binding of Ube2S to the APC/C. Nat Commun 5:3667 (2014).
Sequence Similarity
Belongs to the APC8/CDC23 family.
Post-translational Modification
Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.
Subcellular Location
Cytosol.
Synonyms
ANAPC8 antibody
Anaphase promoting complex subunit 8 antibody
Anaphase-promoting complex subunit 8 antibody
Apc 8 antibody
APC8 antibody
Cdc 23 antibody
CDC23 antibody
CDC23_HUMAN antibody
cell division cycle 23 antibody
Cell division cycle 23 homolog antibody
ExpandImages
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Western blot analysis of CDC23 on different lysates with Rabbit anti-CDC23 antibody (ET1612-84) at 1/5,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: K-562 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Lane 7: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 56 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-84) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"