HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
Catalog# ET1612-69
HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
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WB
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IF-Cell
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IP
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HDAC10 aa 339-388 / 669.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IF-Cell, IP
Target Molecular Weight
Predicted band size: 71 kDa
Positive Control
Rat brain tissue lysate, rat cerebellum tissue lysate, rat pancreas tissue lysate, Hela, A431, A549, human placenta tissue.
Conjugation
unconjugated
Clone Number
SD08-11
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Histone deacetylases (HDACs) play an important role in the modification of chromatin structure and thus in the suppression and activation of transcription and cellular differentiation. There are 11 members in the HDAC family that are divided into four classes. Class I HDACs represent homologs of the yeast histone deacetylase Rpd3, class II HDACs share strong homology with the yeast histone deacetylase Hda1, class III HDACs are closely related to the yeast Sir2 protein and class IV HDACs comprise histone deacetylase 11 (HDAC11)-related enzymes. HDAC10, also known as HD10, is a member of the class II HDACs. It contains an N-terminal Hda1p-related catalytic domain and a unique C-terminal leucine-rich domain. HDAC10 is ubiquitously expressed and can shuttle between the cytoplasm and nucleus in response to celllular signals. It is able to repress transcription and, like other class II HDAC members, its enzymatic activity is inhibited by Trichostatin A (TSA).
Background References
1. Liu J et al. Both HDAC5 and HDAC6 are required for the proliferation and metastasis of melanoma cells. J Transl Med 14:7 (2016).
2. Takase K et al. Monoaminergic and neuropeptidergic neurons have distinct expression profiles of histone deacetylases. PLoS One 8:e58473 (2013).
Sequence Similarity
Belongs to the histone deacetylase family. HD type 2 subfamily.
Tissue Specificity
Widely expressed with high levels in liver and kidney.
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
DKFZP761B039 antibody
HD 10 antibody
HD10 antibody
HDA10_HUMAN antibody
HDAC 10 antibody
Hdac10 antibody
Histone deacetylase 10 antibody
MGC149722 antibody
OTTHUMP00000028555 antibody
Images
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Western blot analysis of HDAC10 on different lysates with Rabbit anti-HDAC10 antibody (ET1612-69) at 1/500 dilution.
Lane 1: Rat brain tissue lysate
Lane 2: Rat cerebellum tissue lysate
Lane 3: Rat pancreas tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 71 kDa
Observed band size: 75 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1612-69) at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining of HDAC10 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of HDAC10 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of HDAC10 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1612-69, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HDAC10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-69, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"