Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [SN70-09]
Catalog# ET1611-81
Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [SN70-09]
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [SN70-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human HBA1 aa 71-120 / 142.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 15 kDa
Positive Control
Human liver tissue lysate, Mouse liver tissue lysate, Rat heart tissue lysate, Rat liver tissue lysate, human lung tissue, human spleen tissue, mouse embryo tissue.
Conjugation
unconjugated
Clone Number
SN70-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:100
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IP
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Use at an assay dependent concentration.
Target
Function
Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The α (16p13.3; 5'-ζ-pseudoz-pseudo α2-pseudo α1-α2-α1-?1-3') and β (11p15.5) globin loci determine the basic hemoglobin structure. The globin portion of hemoglobin consists of two α chains and two β chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between α and β chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the α1-β2 cleavage plane. When the two α1-β2 interfaces are closely bound, hemoglobin has a low affinity for oxygen. Hb A, which contains two α chains plus two β chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two α chains plus two δ chains, and fetal hemoglobin (Hb F), which consists of two α chains together with two γ chains.
Background References
1. Banerjee S et al. Haptoglobin alters oxygenation and oxidation of hemoglobin and decreases propagation of peroxide-induced oxidative reactions. Free Radic Biol Med 53:1317-26 (2012).
2. Shephard F et al. A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson\'s disease. Mitochondrion 14:64-72 (2014).
Sequence Similarity
Belongs to the globin family.
Tissue Specificity
Red blood cells.
Post-translational Modification
The initiator Met is not cleaved in variant Thionville and is acetylated.
Subcellular Location
Cytosol, membrane, extracellular region
Synonyms
Alpha 1 globin antibody
Alpha globin antibody
Alpha one globin antibody
Alpha-globin antibody
HBA_HUMAN antibody
HBA1 antibody
HBA2 antibody
Hemoglobin alpha 1 antibody
Hemoglobin alpha 1 chain antibody
Hemoglobin alpha 1 globin chain antibody
ExpandImages
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Western blot analysis of Hemoglobin subunit alpha on different lysates with Rabbit anti-Hemoglobin subunit alpha antibody (ET1611-81) at 1/1,000 dilution.
Lane 1: Human liver tissue lysate (20 µg/Lane)
Lane 2: Mouse liver tissue lysate (20 µg/Lane)
Lane 3: Rat heart tissue lysate (20 µg/Lane)
Lane 4: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 15 kDa
Observed band size: 13 kDa
Exposure time: Lane 1-2: 25 seconds; Lane 3-4: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-Hemoglobin subunit alpha antibody (ET1611-81) at 1/800 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse embryo tissue using anti-Hemoglobin subunit alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-81, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"