Kv1.1 potassium channel Recombinant Rabbit Monoclonal Antibody [SN66-06]
Catalog# ET1611-7
Kv1.1 potassium channel Recombinant Rabbit Monoclonal Antibody [SN66-06]
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WB
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IHC-P
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IP
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
Kv1.1 potassium channel Recombinant Rabbit Monoclonal Antibody [SN66-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human KCNA1 aa 491-440 / 495.
Species Reactivity
Human, Rat (Predicted: Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P, IP
Target Molecular Weight
Predicted band size: 56 kDa
Positive Control
Human brain tissue lysates, rat brain tissue.
Conjugation
unconjugated
Clone Number
SN66-06
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
Voltage-gated K+ channels in the plasma membrane control the repolarization and the frequency of action potentials in neurons, muscles, and other excitable cells. The KV gene family encodes more than 30 genes that comprise the subunits of the K+ channels, and they vary in their gating and permeation properties, subcellular distribution, and expression patterns. Functional KV channels assemble as tetramers consisting of pore-forming -subunits (KVα), which include the KV1, KV2, KV3, and KV4 proteins, and accessory or KVβ subunits that modify the gating properties of the coexpressed KVα subunits. Differences exist in the patterns of trafficking, biosynthetic processing and surface expression of the major KV1 subunits (KV1.1, KV1.2, KV1.4, KV1.5 and KV1.6) expressed in rat and human brain, suggesting that the individual protein subunits are highly regulated to control for the assembly and formation of functional neuronal channels.
Background References
1. Tomlinson SE et al. Clinical, genetic, neurophysiological and functional study of new mutations in episodic ataxia type 1. J Neurol Neurosurg Psychiatry : (2013).
2. Remke M et al. Adult medulloblastoma comprises three major molecular variants. J Clin Oncol 29:2717-23 (2011).
Sequence Similarity
Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.1/KCNA1 sub-subfamily.
Tissue Specificity
Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level). Detected in the islet of Langerhans.
Post-translational Modification
N-glycosylated.; Palmitoylated on Cys-243; which may be required for membrane targeting.; Phosphorylated on tyrosine residues. Phosphorylation increases in response to NRG1; this inhibits channel activity (By similarity). Phosphorylation at Ser-446 regulates channel activity by down-regulating expression at the cell membrane.
Subcellular Location
Cell membrane, Membrane, Cytoplasmic vesicle, Perikaryon, Endoplasmic reticulum.
Synonyms
AEMK antibody
EA1 antibody
Episodic ataxia with myokymia antibody
HBK1 antibody
HUK1 antibody
Kca1 1 antibody
Kcna1 antibody
KCNA1_HUMAN antibody
Kcpvd antibody
KV1.1 antibody
ExpandImages
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Western blot analysis of Kv1.1 potassium channel on human brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.1 potassium channel antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"