Phospho-MSK1 (S376) Recombinant Rabbit Monoclonal Antibody [SN63-05]
Catalog# ET1611-17
Phospho-MSK1 (S376) Recombinant Rabbit Monoclonal Antibody [SN63-05]
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Rat
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unconjugated
Overview
Product Name
Phospho-MSK1 (S376) Recombinant Rabbit Monoclonal Antibody [SN63-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser376 of Human MSK1 aa 351-400 / 802.
Species Reactivity
Human, Rat
Validated Applications
WB, IF-Cell, IHC-P, IP
Target Molecular Weight
Predicted band size: 90 kDa
Positive Control
HEK-293 cells treated with 200nM PMA for 30 minutes, human colon tissue, rat bladder tissue.
Conjugation
unconjugated
Clone Number
SN63-05
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
Target
Function
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.
Background References
1. Malvi P et al. HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B. Cell Rep Med. 2023 Nov
2. Ning B et al. MSK1 downregulation is involved in inflammatory responses following subarachnoid hemorrhage in rats. Exp Ther Med. 2021 Apr
Sequence Similarity
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Tissue Specificity
Widely expressed with high levels in heart, brain and placenta. Less abundant in lung, kidney and liver.
Post-translational Modification
Ser-376 and Thr-581 phosphorylation is required for kinase activity. Ser-376 and Ser-212 are autophosphorylated by the C-terminal kinase domain, and their phosphorylation is essential for the catalytic activity of the N-terminal kinase domain. Phosphorylated at Ser-360, Thr-581 and Thr-700 by MAPK1/ERK2, MAPK3/ERK1 and MAPK14/p38-alpha. Autophosphorylated at Ser-750, Ser-752 and Ser-758 by the N-terminal kinase domain.; Ubiquitinated.
Subcellular Location
Nucleus, Cytoplasm.
Synonyms
90 kDa ribosomal protein S6 kinase 5 antibody
EC 2.7.11.1 antibody
KS6A5_HUMAN antibody
MGC1911 antibody
Mitogen and stress activated protein kinase 1 antibody
MSPK1 antibody
Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
Nuclear mitogen- and stress-activated protein kinase 1 antibody
Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
Ribosomal protein S6 kinase 90kDa antibody
ExpandImages
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☑ Cell treatment (CT)
Immunocytochemistry analysis of HEK-293 cells treated with or without 200nM PMA for 30 minutes labeling Phospho-MSK1 (S376) with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-MSK1 (S376) antibody (ET1611-17) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-Phospho-MSK1 (S376) antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1611-17, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
