VAV2 Recombinant Rabbit Monoclonal Antibody [SC68-03]
Catalog# ET1610-79
VAV2 Recombinant Rabbit Monoclonal Antibody [SC68-03]
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
VAV2 Recombinant Rabbit Monoclonal Antibody [SC68-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human VAV2 aa 146-194 / 878.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P, IP
Target Molecular Weight
Predicted band size: 101 kDa
Positive Control
Hela cell lysate, SW480 cell lysate, Hela, NIH/3T3, SW480, rat brain tissue, mouse colon tissue.
Conjugation
unconjugated
Clone Number
SC68-03
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
The Vav gene was originally identified on the basis of its oncogenic activation during the course of gene transfer assays. The major translational product of the Vav proto-oncogene has been identified as a protein containing an array of structural motifs. This protein, known as Vav, Vav1 or p95Vav, contains an N-terminal helix-loop-helix domain and a leucine zipper motif similar to that of Myc family proteins that, if deleted, causes oncogenic activation. In addition, Vav contains an SH2 domain, which could indicate its role as a substrate for tyrosine kinases. Expression of Vav is limited exclusively to cells of hematopoietic origin, including those of the erythroid, lymphoid and myeloid lineages. These results suggest that Vav may represent a new type of signal transduction molecule involved in the transduction of tyrosine phosphorylation signaling into transcriptional events. Vav2 is a member of the Vav family of oncoproteins and acts as a guanosine nucleotide exchange factor (GEF) for RhoG and RhoA-like GTPases in a phosphotyrosine-dependent manner.
Background References
1. Jones MC et al. Paxillin kinase linker (PKL) regulates Vav2 signaling during cell spreading and migration. Mol Biol Cell 24:1882-94 (2013).
2. Yin S et al. Vav1-phospholipase C-γ1 (Vav1-PLC-γ1) pathway initiated by T cell antigen receptor (TCRγd) activation is required to overcome inhibition by ubiquitin ligase Cbl-b during γdT cell cytotoxicity. J Biol Chem 288:26448-62 (2013).
Tissue Specificity
Widely expressed.
Post-translational Modification
Phosphorylated on tyrosine residues in response to FGR activation.
Subcellular Location
Cytoplasm.
Synonyms
Guanine nucleotide exchange factor VAV2 antibody
Oncogene VAV2 antibody
Protein vav 2 antibody
VAV 2 antibody
Vav 2 oncogene antibody
VAV-2 antibody
VAV2 antibody
VAV2 oncogene antibody
VAV2_HUMAN antibody
Images
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Western blot analysis of VAV2 on different lysates with Rabbit anti-VAV2 antibody (ET1610-79) at 1/500 dilution.
Lane 1: Hela cell lysate
Lane 2: SW480 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 1 minute;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-79) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining of VAV2 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-79, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of VAV2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-79, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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ICC staining of VAV2 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-79, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-VAV2 antibody (ET1610-79) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-79) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-VAV2 antibody (ET1610-79) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-79) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"