AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667]
Catalog# ET1610-75
AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
AKAP14 Recombinant Rabbit Monoclonal Antibody [SC0667]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human AKAP14 aa 135-170 / 197.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 23 kDa
Positive Control
Jurkat cell lysate, HUVEC cell lysate, A549 cell lysate, DU 145 cell lysate, Mouse testis tissue lysate, Rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
SC0667
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Background References
1. Ross M.T., et al. 2005. The DNA sequence of the human X chromosome. Nature 434:325-337.
2. Kultgen P.L., et al. 2002. Characterization of an A-kinase anchoring protein in human ciliary axonemes. Mol. Biol. Cell 13:4156-4166.
Tissue Specificity
Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.
Subcellular Location
Cytoplasm.
Synonyms
A kinase (PRKA) anchor protein 14 antibody
A kinase anchor protein 14 antibody
A kinase anchor protein 28 kDa antibody
A-kinase anchoring protein 28 antibody
AKAP14 antibody
AKAP28 antibody
PRKA14 antibody
Protein kinase A anchoring protein 14 antibody
Images
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Western blot analysis of AKAP14 on different lysates with Rabbit anti-AKAP14 antibody (ET1610-75) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HUVEC cell lysate
Lane 3: A549 cell lysate
Lane 4: DU 145 cell lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Rat testis tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-75) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"