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MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02]

Usd: 385

Specification

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Catalog# ET1610-70

MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02]

Application

  • WB

  • IHC-P

  • IP

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MEKK2 Recombinant Rabbit Monoclonal Antibody [SC68-02]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human MEKK2 aa 1-47 / 619.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP, FC

Target Molecular Weight

70 kDa

Positive Control

HepG2, Rat brain, HEK293A, MBMEC, mouse brain tissue, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

SC68-02

RRID

AB_3069955

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK 1 activates both MEK-4 and MEK-3/MEK-6 pathways.

Background References

1. Mazur PK et al. SMYD3 links lysine methylation of MAP3K2 to Ras-driven cancer. Nature 510:283-7 (2014).

2. Brown RD et al. MAP kinase kinase kinase-2 (MEKK2) regulates hypertrophic remodeling of the right ventricle in hypoxia-induced pulmonary hypertension. Am J Physiol Heart Circ Physiol 304:H269-81 (2013).

Sequence Similarity

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational Modification

Autophosphorylated.; Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q9Y2U5 Human

SwissProt: Q61083 Mouse

Unigene: 213212 Rat

Synonyms

M3K2_HUMAN antibody

Map3k2 antibody

MAPK/ERK kinase kinase 2 antibody

MEK kinase 2 antibody

MEKK 2 antibody

MEKK2b antibody

Mitogen activated protein kinase kinase kinase 2 antibody

Mitogen-activated protein kinase kinase kinase 2 antibody

Images

  • Western blot analysis of MEKK2 on different lysates with Rabbit anti-MEKK2 antibody (<a href="/products/ET1610-70" style="font-weight: bold;text-decoration: underline;">ET1610-70</a>) at 1/2,000 dilution.<br /><br />Lane 1: Hep G2 (Human hepatocellular carcinoma cell)<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1610-70" style="font-weight: bold;text-decoration: underline;">ET1610-70</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 70 kDa<br />Observed band size: 70 kDa

    Western blot analysis of MEKK2 on different lysates with Rabbit anti-MEKK2 antibody (ET1610-70) at 1/2,000 dilution.

    Lane 1: Hep G2 (Human hepatocellular carcinoma cell)
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1610-70, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

  • <span style="font-weight: bold;">☑ Knockout (KO)</span><br /><br />All lanes: Western blot analysis of MEKK2 with anti-MEKK2 antibody [SC68-02] (<a href="/products/ET1610-70" style="font-weight: bold;text-decoration: underline;">ET1610-70</a>) at 1:1,000 dilution.<br />Lane 1: Wild-type HEK293A whole cell lysate (20 µg).<br />Lane 2: MEKK2 knockout HEK293A whole cell lysate (20 µg).<br />Lane 3: Wild-type MBMEC whole cell lysate (20 µg).<br />Lane 4: MEKK2 knockout MBMEC whole cell lysate (20 µg).<br /><br /><a href="/products/ET1610-70" style="font-weight: bold;text-decoration: underline;">ET1610-70</a> was shown to specifically react with MEKK2 in Wild-type HEK293A and Wild-type MBMEC cells. No band was observed when MEKK2 knockout samples were tested. Wild-type and MEKK2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MEKK2 antibody (<a href="/products/ET1610-70" style="font-weight: bold;text-decoration: underline;">ET1610-70</a>, 1/1,000) and Anti-β-actin antibody (<a href="/products/R1207-1" style="font-weight: bold;text-decoration: underline;">R1207-1</a>, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    ☑ Knockout (KO)

    All lanes: Western blot analysis of MEKK2 with anti-MEKK2 antibody [SC68-02] (ET1610-70) at 1:1,000 dilution.
    Lane 1: Wild-type HEK293A whole cell lysate (20 µg).
    Lane 2: MEKK2 knockout HEK293A whole cell lysate (20 µg).
    Lane 3: Wild-type MBMEC whole cell lysate (20 µg).
    Lane 4: MEKK2 knockout MBMEC whole cell lysate (20 µg).

    ET1610-70 was shown to specifically react with MEKK2 in Wild-type HEK293A and Wild-type MBMEC cells. No band was observed when MEKK2 knockout samples were tested. Wild-type and MEKK2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-MEKK2 antibody (ET1610-70, 1/1,000) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MEKK2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"