GARS Recombinant Rabbit Monoclonal Antibody [SC0651]
Catalog# ET1610-67
GARS Recombinant Rabbit Monoclonal Antibody [SC0651]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
GARS Recombinant Rabbit Monoclonal Antibody [SC0651]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GARS aa 698-739 / 739.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 83 kDa
Positive Control
LNCaP cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, human colon cancer tissue, mouse colon tissue, rat brain tissue.
Conjugation
unconjugated
Clone Number
SC0651
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:200
Target
Function
The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Proteins belonging to this family function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. GlyRS (Glycyl-tRNA synthetase), also known as Glycine-tRNA ligase, is a 739 amino acid class II synthetase that is widely expressed, including in the brain and spinal cord. Defects in the gene encoding GlyRS is the cause of Charcot-Marie-Tooth disease type 2D (CMT2D), which is an autosomal dominant inherited disease characterized by severe weakness, atrophy and absence of deep tendon reflexes in the upper extremities. Defects in the GlyRS gene is also the cause of distal hereditary muscular neuropathy type V (HMN5), a disease similar to CMT2D, though the distal sensory involvement is less severe in HMN5 patients.
Background References
1. Motley WW et al. Charcot-Marie-Tooth-linked mutant GARS is toxic to peripheral neurons independent of wild-type GARS levels. PLoS Genet 7:e1002399 (2011).
2. Vellaichamy A et al. Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases. PLoS One 4:e7075 (2009).
Sequence Similarity
Belongs to the class-II aminoacyl-tRNA synthetase family.
Tissue Specificity
Widely expressed, including in brain and spinal cord.
Subcellular Location
Cytoplasm, Mitochondrion, Cell projection.
Synonyms
AP 4 A synthetase antibody
Charcot Marie Tooth neuropathy 2D antibody
Charcot Marie Tooth neuropathy neuronal type D antibody
CMT2D antibody
Diadenosine tetraphosphate synthetase antibody
DSMAV antibody
EC 6.1.1.14 antibody
Glycine tRNA ligase antibody
Glycyl tRNA synthetase antibody
GlyRS antibody
ExpandImages
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Western blot analysis of GARS on different lysates with Rabbit anti-GARS antibody (ET1610-67) at 1/2,000 dilution.
Lane 1: LNCaP cell lysate (15 µg/Lane)
Lane 2: NIH/3T3 cell lysate (15 µg/Lane)
Lane 3: Mouse colon tissue lysate (30 µg/Lane)
Lane 4: Mouse heart tissue lysate (30 µg/Lane)
Lane 5: Rat brain tissue lysate (30 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 75 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-67) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-GARS antibody (ET1610-67) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-67) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-GARS antibody (ET1610-67) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-67) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-GARS antibody (ET1610-67) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-67) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"