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ATG7 Recombinant Rabbit Monoclonal Antibody [SC06-30]

Usd: 385

Specification

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Catalog# ET1610-53

ATG7 Recombinant Rabbit Monoclonal Antibody [SC06-30]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ATG7 Recombinant Rabbit Monoclonal Antibody [SC06-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human ATG7 aa 654-703 / 703.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Tissue, IHC-P, IP

Target Molecular Weight

Predicted band size: 78 kDa

Positive Control

HeLa cell lysate, Jurkat cell lysate, THP-1 cell lysate, human tonsil tissue, human spleen tissue, human kidney tissue, human liver tissue, rat kidney tissue lysate.

Conjugation

unconjugated

Clone Number

SC06-30

RRID

AB_3069938

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:100-1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Autophagy related 7 is a protein in humans encoded by ATG7 gene. Related to GSA7; APG7L; APG7-LIKE. ATG 7, present in both plant and animal genomes, acts as an essential protein for cell degradation and its recycling. The sequence associates with the ubiquitin- proteasome system, UPS, required for the unique development of an autophagosomal membrane and fusion within cells. ATG7 was identified based on homology to yeast cells Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. The protein appears to be required for fusion of peroxisomal and vacuolar membranes. Autophagy is an important cellular process that helps in maintaining homeostasis. It goes through destroying and recycling the cytoplasmic organelles and macromolecules. During the initiation of autophagy, ATG7 acts like an E-1 enzyme for ubiquitin-like proteins (UBL) such as ATG12 and ATG8. ATG7 helps these UBL proteins in targeting their molecule by binding to them and activating their transfer to an E-2 enzyme. ATG7's role in both of these autophagy-specific UBL systems makes it an essential regulator of autophagosome assembly. Homologous to the ATP-binding and catalytic sites of E1 activator proteins, ATG7 uses its cysteine residue to create a thiol-ester bond with free Ubiquitin molecules. Through UPS, Ubiquitin will continue to bind to other autophagy-related proteins, E2 conjugation proteins and E3 protein ligases, to attach Ubiquitins to a target substrate to induce autophagy. ATG 7 is often associated with ATG12/ ATG5 sequenced ubiquitination cascade. As well in presence of p53 cell cycle pathways during stressed and nutrient poor environments.

Background References

1. Xu Y et al. Deacetylation of ATG7 drives the induction of macroautophagy and LC3-associated microautophagy. Autophagy. 2024 May

2. Yao H et al. Ablation of endothelial Atg7 inhibits ischemia-induced angiogenesis by upregulating Stat1 that suppresses Hif1a expression. Autophagy. 2023 May

Sequence Similarity

Belongs to the ATG7 family.

Tissue Specificity

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Post-translational Modification

Acetylated by EP300.

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: O95352 Human

SwissProt: Q641Y5 Rat

Synonyms

1810013K23Rik antibody

Apg 7 antibody

APG7 autophagy 7 like antibody

APG7 autophagy 7-like (S. cerevisiae) antibody

APG7 like antibody

APG7, S. cerevisiae, homolog of antibody

APG7-like antibody

APG7L antibody

ATG 7 antibody

ATG12-activating enzyme E1 ATG7 antibody

Expand

Images

  • Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: THP-1 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: THP-1 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ATG7 on rat kidney tissue lysates with Rabbit anti-ATG7 antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ATG7 on rat kidney tissue lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/1,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si ATG7 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-53" style="font-weight: bold;text-decoration: underline;">ET1610-53</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ATG7 on different lysates with Rabbit anti-ATG7 antibody (ET1610-53) at 1/2,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si ATG7 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 70 kDa

    Exposure time: 10 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-53) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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