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PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580]

Usd: 385

Specification

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Catalog# ET1610-36

PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580]

Application

  • WB

  • IP

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PI3-kinase p110 subunit beta Recombinant Rabbit Monoclonal Antibody [SC0580]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PIK3CB aa 5-52 / 1070.

Species Reactivity

Human

Validated Applications

WB, IP, IHC-P, FC

Molecular Weight

Predicted band size: 123 kDa

Positive Control

Jurkat cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, human liver carcinoma tissue, Hela.

Conjugation

unconjugated

Clone Number

SC0580

RRID

AB_3069920

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

Target

Function

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. p110 expression is restricted to white blood cells. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ seems to have the capacity to autophosphorylate. p110γ does not interact with the p85 subunits. It has been shown to be activated by α and β γ heterotrimeric G proteins.

Background References

1. H land K et al. Targeting class IA PI3K isoforms selectively impairs cell growth, survival, and migration in glioblastoma. PLoS One 9:e94132 (2014).

2. Wojtalla A et al. Targeting the phosphoinositide 3-kinase p110-a isoform impairs cell proliferation, survival, and tumor growth in small cell lung cancer. Clin Cancer Res 19:96-105 (2013).

Sequence Similarity

Belongs to the PI3/PI4-kinase family.

Tissue Specificity

Expressed ubiquitously.

Post-translational Modification

Phosphorylation at Ser-1070 down-regulates lipid kinase activity.

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P42338 Human

Synonyms

5-bisphosphate 3-kinase 110 kDa catalytic subunit beta antibody

5-bisphosphate 3-kinase catalytic subunit beta isoform antibody

DKFZp779K1237 antibody

MGC133043 antibody

OTTHUMP00000216901 antibody

OTTHUMP00000216904 antibody

p110 BETA antibody

p110Beta antibody

Phosphatidylinositol 3 kinase catalytic beta polypeptide antibody

Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta antibody

Expand

Images

  • Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: MCF7 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 123 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (ET1610-36) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: MCF7 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 123 kDa
    Observed band size: 110 kDa

    Exposure time: 1 minute; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-PI3-kinase p110 subunit beta KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 123 kDa<br />Observed band size: 110 kDa<br /><br />Exposure time: 40 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PI3-kinase p110 subunit beta on different lysates with Rabbit anti-PI3-kinase p110 subunit beta antibody (ET1610-36) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-PI3-kinase p110 subunit beta KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 123 kDa
    Observed band size: 110 kDa

    Exposure time: 40 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-36) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI3-kinase p110 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-PI3-kinase p110 subunit beta antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1610-36" style="font-weight: bold;text-decoration: underline;">ET1610-36</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PI3-kinase p110 subunit beta was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-36, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation