Skip to content

Phospho-TrkB (Y817) Recombinant Rabbit Monoclonal Antibody [SC0556]

Usd: 205

Specification

Bulk Order Quote | Customization Request

Catalog# ET1610-35

Phospho-TrkB (Y817) Recombinant Rabbit Monoclonal Antibody [SC0556]

Application

  • WB

  • IF-Cell

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-TrkB (Y817) Recombinant Rabbit Monoclonal Antibody [SC0556]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Tyr817 of Human TrkB aa 789-822 / 822.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 92 kDa

Positive Control

Mouse brain tissue lysate, rat brain tissue lysate, PANC-1, SH-SY5Y, human brain tissue, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

SC0556

RRID

AB_3069919

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • IF-Cell

  • 1:50-1:200

  • IF-Tissue

  • 1:200

  • IHC-P

  • 1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on tyrosine of Trk B gp145, one of the receptors encoded by NTRK2, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3.

Background References

1. Nannini M et al. Integrated genomic study of quadruple-WT GIST (KIT/PDGFRA/SDH/RAS pathway wild-type GIST). BMC Cancer 14:685 (2014).

2. Zhang HH et al. The BDNF/TrkB signaling pathway is involved in heat hyperalgesia mediated by Cdk5 in rats. PLoS One 9:e85536 (2014).

Sequence Similarity

Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.

Tissue Specificity

Isoform TrkB is expressed in the central and peripheral nervous system. In the central nervous system (CNS), expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophthalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is mainly expressed in the brain but also detected in other tissues including pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in the brain.

Post-translational Modification

Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated.; Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity).

Subcellular Location

Cell membrane, Endosome membrane.

UNIPROT

SwissProt: Q16620 Human

SwissProt: P15209 Mouse

SwissProt: Q63604 Rat

Synonyms

AI848316 antibody

BDNF tropomyosine receptor kinase B antibody

BDNF/NT 3 growth factors receptor antibody

BDNF/NT-3 growth factors receptor antibody

Brain derived neurotrophic factor receptor antibody

C030027L06Rik antibody

EC 2.7.10.1 antibody

GP145 TrkB antibody

GP145-TrkB antibody

GP145-TrkB/GP95-TrkB antibody

Expand

Images

  • Western blot analysis of Phospho-TrkB (Y817) on different lysates with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 92 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Phospho-TrkB (Y817) on different lysates with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 92 kDa
    Observed band size: 130 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-35) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • ICC staining of Phospho-TrkB (Y817) in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Phospho-TrkB (Y817) in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • ICC staining of Phospho-TrkB (Y817) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Phospho-TrkB (Y817) in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-35, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  • Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded human liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1610-35" style="font-weight: bold;text-decoration: underline;">ET1610-35</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Relative expression (RE)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Rabbit anti-Phospho-TrkB (Y817) antibody (ET1610-35) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-35) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

TrkB Recombinant Rabbit Monoclonal Antibody [JE58-22]

Application: WB,IHC-P,IHC-Fr

Reactivity: Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-TrkB (Y817) Recombinant Rabbit Monoclonal Antibody [SC0556] - BSA and Azide free

Application: WB,IF-Cell,IF-Tissue,IHC-P,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated