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nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

Usd: 385

Specification

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Catalog# ET1609-61

nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

Application

  • WB

  • IF-Tissue

  • IHC-P

  • IP

Reactivity

  • Human

  • Mouse

  • Rat

Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

nNOS Recombinant Rabbit Monoclonal Antibody [ST520]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human nNOS aa 1398-1434 / 1434.

Species Reactivity

Human, Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IF-Tissue, IHC-P, IP

Molecular Weight

Predicted band size: 161 kDa

Positive Control

Mouse brain tissue lysate, Mouse hippocampus tissue lysate, Mouse cerebellum tissue lysate, Rat hippocampus tissue lysate, Rat cerebellum tissue lysate, human skeletal muscle tissue, rat brain tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue.

Conjugation

unconjugated

Clone Number

ST520

RRID

AB_3069870

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:200-1:1,000

  • IP

  • Use at an assay dependent concentration.

Target

Function

Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Background References

1. Khan M et al. Blocking a vicious cycle nNOS/peroxynitrite/AMPK by S-nitrosoglutathione: implication for stroke therapy. BMC Neurosci 16:42 (2015).

2. Li G et al. Firing of hippocampal neurogliaform cells induces suppression of synaptic inhibition. J Neurosci 34:1280-92 (2014).

Sequence Similarity

Belongs to the NOS family.

Tissue Specificity

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.

Post-translational Modification

Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).

Subcellular Location

Cell membrane, sarcolemma, Cell projection, dendritic spine.

UNIPROT

SwissProt: P29475 Human

SwissProt: Q9Z0J4 Mouse

SwissProt: P29476 Rat

Synonyms

2310005C01Rik antibody

BNOS antibody

Constitutive NOS antibody

EC 1.14.13.39 antibody

IHPS 1 antibody

IHPS1 antibody

N-NOS antibody

NC-NOS antibody

neuronal Nitric Oxide Synthase antibody

Neuronal NOS antibody

Expand

Images

  • Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/1,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 2: Mouse hippocampus tissue lysate (40 µg/Lane)<br />Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)<br />Lane 4: Rat hippocampus tissue lysate (40 µg/Lane)<br />Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 161 kDa<br />Observed band size: 161 kDa<br /><br />Exposure time: 1 minute; ECL: K1802;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of nNOS on different lysates with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution.

    Lane 1: Mouse brain tissue lysate (40 µg/Lane)
    Lane 2: Mouse hippocampus tissue lysate (40 µg/Lane)
    Lane 3: Mouse cerebellum tissue lysate (40 µg/Lane)
    Lane 4: Rat hippocampus tissue lysate (40 µg/Lane)
    Lane 5: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 161 kDa
    Observed band size: 161 kDa

    Exposure time: 1 minute; ECL: K1802;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-61) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-nNOS antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-61" style="font-weight: bold;text-decoration: underline;">ET1609-61</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-nNOS antibody (ET1609-61) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-61) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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metafield image

nNOS Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC,IHC-Fr

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated