Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
Catalog# ET1609-55
Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
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WB
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IP
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
Phospho-BTK (Y223) Recombinant Rabbit Monoclonal Antibody [ST51-05]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Tyr223 of human BTK.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB, IP
Molecular Weight
Predicted band size: 76 kDa
Positive Control
Ramos cell lysate, Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, K562 cells treated with pervanadate.
Conjugation
unconjugated
Clone Number
ST51-05
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000-1:2,000
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IP
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Use at an assay dependent concentration.
Target
Function
The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton’s disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.
Background References
1. Guo W et al. Targeting Btk/Etk of prostate cancer cells by a novel dual inhibitor. Cell Death Dis 5:e1409 (2014).
2. Dong S et al. IPI-145 antagonizes intrinsic and extrinsic survival signals in chronic lymphocytic leukemia cells. Blood 124:3583-6 (2014).
Sequence Similarity
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Tissue Specificity
Predominantly expressed in B-lymphocytes.
Post-translational Modification
Following B-cell receptor (BCR) engagement, translocates to the plasma membrane where it gets phosphorylated at Tyr-551 by LYN and SYK. Phosphorylation at Tyr-551 is followed by autophosphorylation of Tyr-223 which may create a docking site for a SH2 containing protein. Phosphorylation at Ser-180 by PRKCB, leads in translocation of BTK back to the cytoplasmic fraction. Phosphorylation at Ser-21 and Ser-115 creates a binding site for PIN1 at these Ser-Pro motifs, and promotes it's recruitment.
Subcellular Location
Cytoplasm, Nucleus, Cell membrane.
Synonyms
Agammaglobulinaemia tyrosine kinase antibody
AGMX 1 antibody
AGMX1 antibody
AT antibody
ATK antibody
B cell progenitor kinase antibody
B-cell progenitor kinase antibody
BPK antibody
Bruton agammaglobulinemia tyrosine kinase antibody
Bruton tyrosine kinase antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-BTK (Y223) on different lysates with Rabbit anti-Phospho-BTK (Y223) antibody (ET1609-55) at 1/1,000 dilution.
Lane 1: Ramos cell lysate
Lane 2: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate
Lane 3: Ramos treated with 12μg/mL human IgM for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 76 kDa
Observed band size: 76 kDa
Exposure time: 59 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-55) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Cell treatment (CT)
Western blot analysis of Phospho-BTK(Y223) on different lysates using anti-Phospho-BTK(Y223) antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562 cells treated with pervanadate
Lane 2: Untreated K562 cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
BTK Recombinant Rabbit Monoclonal Antibody [SN69-07] - BSA and Azide free
Application: WB,IHC-P,IP,IF-Cell
Reactivity: Human,Mouse
Conjugate: unconjugated
