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Phospho-GATA3 (S308) Recombinant Rabbit Monoclonal Antibody [ST44-09]

Usd: 385

Specification

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Catalog# ET1609-17

Phospho-GATA3 (S308) Recombinant Rabbit Monoclonal Antibody [ST44-09]

Application

  • WB

  • IHC-P

  • IP

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-GATA3 (S308) Recombinant Rabbit Monoclonal Antibody [ST44-09]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser308 of Human GATA3 aa 281-330 / 443.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IP, IF-Tissue

Target Molecular Weight

Predicted band size: 48 kDa

Positive Control

Jurkat cell lysate, human skin tissue lysate, human stomach tissue, mouse stomach tissue, rat stomach tissue.

Conjugation

unconjugated

Clone Number

ST44-09

RRID

AB_3069830

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

  • IP

  • Use at an assay dependent concentration.

  • IF-Tissue

  • 1:50

Target

Function

GATA-3 antibody (GATA binding protein 3) is a member of the GATA family of transcription factors. GATA-3 appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 has a strong association with estrogen receptor-alpha expression in breast cancer and evidence exists that GATA-3 may be used to predict response to hormonal therapy of breast cancer patients. GATA-3 has also been shown to be a novel marker for bladder cancer. In one study, GATA-3 stained 67% of 308 urothelial carcinomas but no prostate or renal carcinomas.

Background References

1. Kim TH et al. Identification of Creb3l4 as an essential negative regulator of adipogenesis. Cell Death Dis 5:e1527 (2014).

2. Izzo F et al. Progesterone receptor activation downregulates GATA3 by transcriptional repression and increased protein turnover promoting breast tumor growth. Breast Cancer Res 16:491 (2014).

Tissue Specificity

T-cells and endothelial cells.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P23771 Human

SwissProt: P23772 Mouse

Entrez Gene: 85471 Rat

Synonyms

GATA 3 antibody

GATA binding factor 3 antibody

GATA binding protein 3 antibody

GATA-binding factor 3 antibody

Gata3 antibody

GATA3_HUMAN antibody

HDR antibody

HDRS antibody

MGC2346 antibody

MGC5199 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-GATA3 (S308) on different lysates with Rabbit anti-Phospho-GATA3 (S308) antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/2,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Jurkat cell lysate, treated with λpp for 1 hour<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 48 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:100,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-GATA3 (S308) on different lysates with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/2,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Jurkat cell lysate, treated with λpp for 1 hour

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1609-17) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1609-17" style="font-weight: bold;text-decoration: underline;">ET1609-17</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue with Rabbit anti-Phospho-GATA3 (S308) antibody (ET1609-17) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-17) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Application: IF-Tissue<br /><br />Species: Mouse<br /><br />Site: stomach<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/50

    Application: IF-Tissue

    Species: Mouse

    Site: stomach

    Sample: Paraffin-embedded section

    Antibody concentration: 1/50

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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