Skip to content

5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# ET1608-37

5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

5T4 Recombinant Rabbit Monoclonal Antibody [SU34-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human 5T4 aa 381-420 / 420.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, IP, IHC-P

Target Molecular Weight

Predicted band size: 46 kDa

Positive Control

MCF7 cell lysate, Human lung tissue lysate, human placenta tissue.

Conjugation

unconjugated

Clone Number

SU34-05

RRID

AB_3069803

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IP

  • Use at an assay dependent concentration.

  • IHC-P

  • 1:1,000

Target

Function

Trophoblast glycoprotein, also known as TPBG, 5T4, Wnt-Activated Inhibitory Factor 1 or WAIF1, is a human protein encoded by a TPBG gene. TPBG is an antagonist of Wnt/β-catenin signalling pathway. 5T4 is an antigen expressed in a number of carcinomas. 5T4 is often referred to as an oncofetal antigen due to its expression in foetal trophoblast (where it was first discovered) or trophoblast glycoprotein (TPBG). 5T4 is found in tumors including the colorectal, ovarian, and gastric. Its expression is used as a prognostic aid in these cases. It has very limited expression in normal tissue but is widespread in malignant tumours throughout their development.

Background References

1. Patterson KG et al. Control of established colon cancer xenografts using a novel humanized single chain antibody-streptococcal superantigen fusion protein targeting the 5T4 oncofetal antigen. PLoS One 9:e95200 (2014).

2. Sapra P et al. Long-term tumor regression induced by an antibody-drug conjugate that targets 5T4, an oncofetal antigen expressed on tumor-initiating cells. Mol Cancer Ther 12:38-47 (2013).

Tissue Specificity

Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

Post-translational Modification

Highly glycosylated.

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q13641 Human

SwissProt: Q9Z0L0 Mouse

SwissProt: Q5PQV5 Rat

Synonyms

5T4 antibody

5T4 oncofetal antigen antibody

5T4 oncofetal trophoblast glycoprotein antibody

5T4 oncotrophoblast glycoprotein antibody

5T4AG antibody

AW495680 antibody

M6P1 antibody

TPBG antibody

TPBG_HUMAN antibody

Trophoblast glycoprotein antibody

Images

  • Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/1,000 dilution.<br /><br />Lane 1: MCF7 cell lysate (20 µg/Lane)<br />Lane 2: Human lung tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 46 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution.

    Lane 1: MCF7 cell lysate (20 µg/Lane)
    Lane 2: Human lung tissue lysate (40 µg/Lane)

    Predicted band size: 46 kDa
    Observed band size: 75 kDa

    Exposure time: Lane 1: 25 seconds; Lane 2: 2 minutes 6 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-5T4 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 46 kDa<br />Observed band size: 75 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of 5T4 on different lysates with Rabbit anti-5T4 antibody (ET1608-37) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-5T4 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 46 kDa
    Observed band size: 75 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-37) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-5T4 antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1608-37" style="font-weight: bold;text-decoration: underline;">ET1608-37</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-5T4 antibody (ET1608-37) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-37) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"