ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26]
Catalog# ET1608-20
ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26]
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WB
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IHC-P
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Human
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Rat
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Mouse
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unconjugated
Overview
Product Name
ABCG1 Recombinant Rabbit Monoclonal Antibody [SU03-26]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ABCG1 aa 161-210 / 678.
Species Reactivity
Human (Predicted: Rat, Mouse)
Predicted species support after-sales service
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 76 kDa
Positive Control
THP-1 cell lysate, MCF-7 cell lysate, human lung tissue, human skin tissue.
Conjugation
unconjugated
Clone Number
SU03-26
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
Target
Function
ATP-binding cassette sub-family G member 1 is a protein that in humans is encoded by the ABCG1 gene. It is a homolog of the well-known Drosophila gene white. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage, cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.
Background References
1. Hu YW et al. A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis. J Lipid Res 55:681-97 (2014).
2. Adlakha YK et al. Pro-apoptotic miRNA-128-2 modulates ABCA1, ABCG1 and RXRa expression and cholesterol homeostasis. Cell Death Dis 4:e780 (2013).
Sequence Similarity
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Tissue Specificity
Expressed in several tissues. Expressed in macrophages; expression is increased in macrophages from patients with Tangier disease.
Post-translational Modification
Palmitoylation at Cys-315 seems important for trafficking from the endoplasmic reticulum.
Subcellular Location
Endoplasmic reticulum membrane, Golgi apparatus membrane.
Synonyms
ABC transporter 8 antibody
ABC8 antibody
ABCG1 antibody
ABCG1_HUMAN antibody
ATP-binding cassette sub family G member 1 antibody
ATP-binding cassette sub-family G member 1 antibody
ATP-binding cassette transporter 8 antibody
ATP-binding cassette transporter member 1 of subfamily G antibody
ATP-binding cassette, sub family G WHITE member 1 antibody
homolog of Drosophila white antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-ABCG1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1608-20, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Western blot analysis of ABCG1 on MCF7 cell lysate with Rabbit anti-ABCG1 antibody (ET1608-20) at 1/2000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1608-20, 1/2000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 75.6 kDa
Observed band size: 100 kDa -
Western blot analysis of ABCG1 on THP-1 cell lysate with Rabbit anti-ABCG1 antibody (ET1608-20) at 1/2000 dilution.
Lysates/proteins at 15 µg/Lane.
Exposure time: 59 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ET1608-20, 1/2000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 75.6 kDa
Observed band size: 100 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"