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Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]

Usd: 385

Specification

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Catalog# ET1607-16

Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]

Application

  • WB

  • IP

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Caveolin-3 Recombinant Rabbit Monoclonal Antibody [SY22-06]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Caveolin-3 aa 1-125 / 151.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IP, IHC-P

Target Molecular Weight

17 kDa

Positive Control

Rat heart tissue, human striated muscle tissue, HEK-293 cell lysate, Mouse heart tissue lysate, Mouse skeletal muscle tissue lysate, Rat heart tissue lysate, Rat skeletal muscle tissue lysate.

Conjugation

unconjugated

Clone Number

SY22-06

RRID

AB_3069753

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:1,000

Target

Function

Caveolae (also known as plasmalemmal vesicles) are 50-100 nM flask-shaped membranes that represent a subcompartment of the plasma membrane. On the basis of morphological studies, caveolae have been implicated to function in the transcytosis of various macromolecules (including LDL) across capillary endothelial cells, uptake of small molecules via potocytosis and the compartmentalization of certain signaling molecules including G protein-coupled receptors. Three proteins, caveolin-1, caveolin-2 and caveolin-3, have been identified as principal components of caveolae. Two forms of caveolin-1, designated alpha and beta, share a distinct but overlapping cellular distribution and differ by an amino terminal 31 amino acid sequence which is absent from the beta isoform. Caveolin-1 shares 31% identity with caveolin-2 and 65% identity with caveolin-3 at the amino acid level. Functionally, the three proteins differ in their interactions with heterotrimeric G protein isoforms.

Background References

1. Niesman IR et al. Traumatic brain injury enhances neuroinflammation and lesion volume in caveolin deficient mice. J Neuroinflammation 11:39 (2014).

2. Zheng J et al. Chymase Mediates Injury and Mitochondrial Damage in Cardiomyocytes during Acute Ischemia/Reperfusion in the Dog. PLoS One 9:e94732 (2014).

Sequence Similarity

Belongs to the caveolin family.

Tissue Specificity

Expressed predominantly in muscle.

Post-translational Modification

Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2.

Subcellular Location

Golgi apparatus membrane, Cell membrane, Membrane.

UNIPROT

SwissProt: P56539 Human

SwissProt: P51637 Mouse

SwissProt: P51638 Rat

Synonyms

CAV3 antibody

CAV3_HUMAN antibody

Caveolin 3 antibody

Caveolin-3 antibody

LGMD1C antibody

LQT9 antibody

M-caveolin antibody

MGC126100 antibody

MGC126101 antibody

MGC126129 antibody

Expand

Images

  • Western blot analysis of Caveolin-3 on different lysates with Rabbit anti-Caveolin-3 antibody (<a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>) at 1/2,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate <br />Lane 2: Mouse heart tissue lysate <br />Lane 3: Mouse skeletal muscle tissue lysate <br />Lane 4: Rat heart tissue lysate <br />Lane 5: Rat skeletal muscle tissue lysate <br /><br />Lysates/proteins at 30 µg/Lane.<br />Exposure time: 6 seconds; ECL: K1801<br /><br />Negative expression of Caveolin-3 protein in HEK-293 cell is consistent with the predicted expression pattern.<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 17 kDa<br />Observed band size: 17 kDa

    Western blot analysis of Caveolin-3 on different lysates with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/2,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: Mouse heart tissue lysate
    Lane 3: Mouse skeletal muscle tissue lysate
    Lane 4: Rat heart tissue lysate
    Lane 5: Rat skeletal muscle tissue lysate

    Lysates/proteins at 30 µg/Lane.
    Exposure time: 6 seconds; ECL: K1801

    Negative expression of Caveolin-3 protein in HEK-293 cell is consistent with the predicted expression pattern.

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ET1607-16, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 17 kDa
    Observed band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caveolin-3 antibody (<a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Caveolin-3 antibody (<a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1607-16" style="font-weight: bold;text-decoration: underline;">ET1607-16</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human striated muscle tissue with Rabbit anti-Caveolin-3 antibody (ET1607-16) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-16) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"