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CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01]

Usd: 385

Specification

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Catalog# ET1607-1

CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01]

Application

  • WB

  • FC

Reactivity

  • Human

Predicted reactivity

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CDC7 Kinase Recombinant Rabbit Monoclonal Antibody [SY21-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CDC7 Kinase aa 1-50 / 574.

Species Reactivity

Human (Predicted: Mouse, Rat)

Predicted species support after-sales service

Validated Applications

WB, FC

Target Molecular Weight

64 kDa

Positive Control

Jurkat cell lysate, Hela cell lysate, A431.

Conjugation

unconjugated

Clone Number

SY21-01

RRID

AB_3069750

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

Target

Function

Cell division cycle 7-related protein kinase is an enzyme that in humans is encoded by the CDC7 gene. The Cdc7 kinase is involved in regulation of the cell cycle at the point of chromosomal DNA replication. The gene CDC7 appears to be conserved throughout eukaryotic evolution; this means that most eukaryotic cells have the Cdc7 kinase protein. The product encoded by this gene is predominantly localized in the nucleus and is a cell division cycle protein with kinase activity. The protein is a serine-threonine kinase that is activated by another protein called either Dbf4 in the yeast Saccharomyces cerevisiae or ASK in mammals. The Cdc7/Dbf4 complex adds a phosphate group to the minichromosome maintenance (MCM) protein complex allowing for the initiation of DNA replication in mitosis (as explained in the Cdc7 and Replication section below). Although expression levels of the protein appear to be constant throughout the cell cycle, the protein kinase activity appears to increase during S phase. It has been suggested that the protein is essential for initiation of DNA replication and that it plays a role in regulating cell cycle progression. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Additional transcript sizes have been detected, suggesting the presence of alternative splicing.

Background References

1. Cao, JX. et al. 2014. MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells. Cell Death Dis. 5: e1426.

2. Georges, SA. et al. 2008. Coordinated regulation of cell cycle transcripts by p53-Inducible microRNAs, miR-192 and miR-215. Cancer Res.. 68: 10105-10112.

Sequence Similarity

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O00311 Human

SwissProt: Q9Z0H0 Mouse

Unigene: 20842 Rat

Synonyms

Cdc 7 antibody

CDC7 antibody

CDC7 cell division cycle 7 antibody

CDC7 cell division cycle 7 like 1 antibody

CDC7 L1 antibody

Cdc7 like 1 antibody

CDC7 related kinase antibody

CDC7-related kinase antibody

CDC7_HUMAN antibody

CDC7L 1 antibody

Expand

Images

  • Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ET1607-1" style="font-weight: bold;text-decoration: underline;">ET1607-1</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Jurkat cell lysate<br />Lane 2: Hela cell lysate

    Western blot analysis of CDC7 Kinase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Jurkat cell lysate
    Lane 2: Hela cell lysate

  • Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ET1607-1" style="font-weight: bold;text-decoration: underline;">ET1607-1</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CDC7 Kinase was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1607-1, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"