GAP43 Rabbit Polyclonal Antibody (ER40201)
Catalog# ER40201
Rabbit Polyclonal to GAP43
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ER40201) at 1/100,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A549 cell lysate (negative)
Lane 3: Neuro-2a cell lysate
Lane 4: Mouse lung tissue lysate (negative)
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 43/45 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40201) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
GAP43 Rabbit Polyclonal Antibody (ER40201)
Immunogen
Synthetic peptide within mouse gap43 aa 178-227 / 227.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, mouse brain tissue lysate, rat brain tissue lysate, Neuro-2a, rat brain tissue, mouse brain tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:100,000
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IF-Cell
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1:200
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IHC-P
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1:1,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 25 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GAP43
SYNONYMS
Axonal membrane protein GAP 43 antibody;Axonal membrane protein GAP-43 antibody;B 50 antibody;Calmodulin binding protein P 57 antibody;F1 antibody;GAP 43 antibody;GAP43 antibody;Growth Associated Protein 43 antibody;Growth-associated protein 43 antibody;Nerve Growth Related Peptide antibody;Nerve growth related peptide GAP43 antibody;NEUM_HUMAN antibody;Neural phosphoprotein B 50 antibody;Neural phosphoprotein B-50 antibody;Neuromodulin antibody;Neuron growth associated protein 43 antibody;PP46 antibody;Protein F1 antibody;QtrA-11580 antibody;QtrA-13071 antibody
SEQUENCE SIMILARITIES
Belongs to the neuromodulin family.
TISSUE SPECIFICITY
Expressed in the hippocampus (at protein level). Expressed in the dorsal root ganglion and the spinal cord (at protein level).
POST-TRANSLATIONAL MODIFICATION
Phosphorylated. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity (By similarity).; Palmitoylated by ZDHHC3. Palmitoylation is regulated by ARF6 and is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 (By similarity).
SUBCELLULAR LOCATION
Cell membrane, Cell projection, Cytoplasm, Membrane, Synapse.
FUNCTION
GAP43, is a nervous tissue-specific cytoplasmic protein that can be attached to the membrane via a dual palmitoylation sequence on cysteines 3 and 4. This sequence targets GAP43 to lipid rafts. It is a major protein kinase C (PKC) substrate and is considered to play a key role in neurite formation, regeneration, and plasticity. The role of GAP-43 in CNS development is not limited to effects on axons: It is also a component of the centrosome, and differentiating neurons that do not express GAP-43 show mislocalization of the centrosome and mitotic spindles, particularly in neurogenic cell divisions. As a consequence, in the cerebellum, the neuronal precursor pool fails to expand normally and the cerebellum is significantly smaller.
CITATIONS
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sudan
GAP43,a novel metastasis promoter in non-small cell lung cancer
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WB
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IHC
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Human
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Citation
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