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ROCK1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER40122

ROCK1 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

ROCK1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within residues of ROCK1 aa 1,305-1,354 / 1,354.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 158 kDa

Positive Control

A431 cell lysate, Human lung tissue lysate, Mouse spleen tissue lysate, C6 cell lysate, L6 cell lysate, HAP1 cell lysate, human colon cancer tissue, rat brain tissue.

Conjugation

unconjugated

RRID

AB_3069574

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:200

Target

Function

ROCK1 is a protein serine/threonine kinase also known as rho-associated, coiled-coil-containing protein kinase 1. ROCK1 has a ubiquitous tissue distribution, but subcellularly it is thought to colocalize with the centrosomes. This is consistent with its function as a key modulator of cell motility, tumor cell invasion, and actin cytoskeleton organization. ROCK1 has a diverse range of functions in the body. It is a key regulator of actin-myosin contraction, stability, and cell polarity. These contribute to many progresses such as regulation of morphology, gene transcription, proliferation, differentiation, apoptosis and oncogenic transformation. Other functions involve smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility. These functions are activated by phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A.

Background References

1. "Identification of ROCK1 as an upstream activator of the JIP-3 to JNK signaling axis in response to UVB damage." Ongusaha P.P., Qi H.H., Raj L., Kim Y.B., Aaronson S.A., Davis R.J., Shi Y., Liao J.K., Lee S.W. Sci. Signal. 1:RA14-RA14(2008)

2. "Distinct roles for ROCK1 and ROCK2 in the regulation of keratinocyte differentiation." Lock F.E., Hotchin N.A. PLoS ONE 4:E8190-E8190(2009)

Sequence Similarity

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Tissue Specificity

Detected in blood platelets.

Post-translational Modification

Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

Subcellular Location

Cytoplasm, cell membrane, Nucleus, Cell projection, Cytoskeleton, Golgi apparatus, Membrane.

UNIPROT

SwissProt: Q13464 Human

SwissProt: P70335 Mouse

SwissProt: Q63644 Rat

Synonyms

coiled-coil-containing protein kinase 1 antibody

coiled-coil-containing protein kinase I antibody

MGC131603 antibody

MGC43611 antibody

p160 Rhoassociated coiled coil-forming protein kinase antibody

p160 ROCK-1 antibody

p160 ROCK1 antibody

p160ROCK antibody

PRO0435 antibody

Renal carcinoma antigen NY REN 35 antibody

Expand

Images

  • Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: Human lung tissue lysate<br />Lane 3: Mouse spleen tissue lysate<br />Lane 4: C6 cell lysate<br />Lane 5: L6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 158 kDa<br />Observed band size: 158 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/1,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: Human lung tissue lysate
    Lane 3: Mouse spleen tissue lysate
    Lane 4: C6 cell lysate
    Lane 5: L6 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 158 kDa
    Observed band size: 158 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-ROCK1 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 158 kDa<br />Observed band size: 158 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ROCK1 on different lysates with Rabbit anti-ROCK1 antibody (ER40122) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-ROCK1 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 158 kDa
    Observed band size: 158 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40122) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ROCK1 antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ROCK1 antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER40122" style="font-weight: bold;text-decoration: underline;">ER40122</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-ROCK1 antibody (ER40122) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER40122) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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