MGMT Rabbit Polyclonal Antibody (ER40104)
Catalog# ER40104
Rabbit Polyclonal to MGMT
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WB
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IF-Cell
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IHC-P
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FC
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Human
Western blot analysis of MGMT on different lysates with Rabbit anti-MGMT antibody (ER40104) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-MGMT KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER40104) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
MGMT Rabbit Polyclonal Antibody (ER40104)
Immunogen
Synthetic peptide within residues of mgmt aa 158-207 / 207.
Host
Rabbit
Positive Control
MCF-7 cell lysate, Hela cell lysate, Dauai cell lysate, Raji cell lysate, SK-BR-3 cell lysate, SKOV3 cell lysate, human kidney tissue lysate, human liver tissue lysate, human thymus tissue lysate, human placentae tissue lysate, A172, SK-BR-3, MCF-7, human tonsil tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:200
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IHC-P
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1:200
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FC:1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MGMT
SYNONYMS
6 O methylguanine DNA methyltransferase antibody;6-O-methylguanine-DNA methyltransferase antibody;Agat antibody;AGT antibody;AI267024 antibody;EC 2.1.1.63 antibody;Methylated DNA protein cysteine methyltransferase antibody;Methylated-DNA--protein-cysteine methyltransferase antibody;Methylguanine DNA methyltransferase antibody;MGC107020 antibody;MGMT antibody;MGMT_HUMAN antibody;O 6 methylguanine DNA alkyltransferase antibody;O 6 methylguanine DNA methyltransferase antibody;O-6-methylguanine-DNA methyltransferase antibody;O-6-methylguanine-DNA-alkyltransferase antibody
SEQUENCE SIMILARITIES
Belongs to the MGMT family.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O (6) position of guanine, restoring guanine to its normal form without causing DNA breaks. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is ubiquitously expressed in normal human tissues and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy.
CITATIONS
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Pei, J., Su, Z., Zeng, X., Zhong, Y., Zhang, Y., Yang, Y., Lu, Q., Li, J., & Deng, Y.
Protocatechuic aldehyde acts synergistically with dacarbazine to augment DNA double-strand breaks and promote apoptosis in cutaneous melanoma cells
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WB
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Citation
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