MVK Rabbit Polyclonal Antibody
Catalog# ER2001-64
MVK Rabbit Polyclonal Antibody
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WB
-
FC
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Human
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unconjugated
Overview
Product Name
MVK Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human MVK aa 200-396.
Species Reactivity
Human
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HeLa (Human cervical adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, A431 (Human epidermoid carcinoma skin squamous cell) cell lysate, HepG2.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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FC
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1:50-1:100
Target
Function
This gene encodes the peroxisomal enzyme mevalonate kinase. Mevalonate is a key intermediate, and mevalonate kinase a key early enzyme, in isoprenoid and sterol synthesis. Mevalonate kinase deficiency caused by mutation of this gene results in mevalonic aciduria, a disease characterized psychomotor retardation, failure to thrive, hepatosplenomegaly, anemia and recurrent febrile crises. Defects in this gene also cause hyperimmunoglobulinaemia D and periodic fever syndrome, a disorder characterized by recurrent episodes of fever associated with lymphadenopathy, arthralgia, gastrointestinal dismay and skin rash. Alternative splicing results in multiple transcript variants.
Background References
1. Hinson D.D. et. al. Post-translational regulation of mevalonate kinase by intermediates of the cholesterol and nonsterol isoprene biosynthetic pathways. J. Lipid Res. 38:2216-2223(1997).
Subcellular Location
Cytoplasm, Peroxisome.
UNIPROT
Synonyms
FLJ96772 antibody
KIME_HUMAN antibody
LH receptor mRNA binding protein antibody
LRBP antibody
Mevalonate kinase 1 antibody
Mevalonate kinase antibody
Mevalonic aciduria antibody
MK antibody
mvk antibody
MVLK antibody
ExpandImages
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Western blot analysis of MVK on different lysates with Rabbit anti-MVK antibody (ER2001-64) at 1/2,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 3: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-64, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 42 kDa
Observed band size: 45 kDa -
Flow cytometric analysis of MVK was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-64, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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