Skip to content

eIF-6 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# ER2001-54

eIF-6 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

eIF-6 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human eIF-6 aa 1-200.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

26 kDa

Positive Control

SHSY5Y cell lysate, A549 cell lysate, HepG2 cell lysate, human colon tissue, mouse colon tissue, SW620.

Conjugation

unconjugated

RRID

AB_3069531

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

Target

Function

Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. Behaves as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (RACK1)-dependent protein kinase C activity. In tissues responsive to insulin, controls fatty acid synthesis and glycolysis by exerting translational control of adipogenic transcription factors such as CEBPB, CEBPD and ATF4 that have G/C rich or uORF in their 5'UTR. Required for ROS-dependent megakaryocyte maturation and platelets formation, controls the expression of mitochondrial respiratory chain genes involved in reactive oxygen species (ROS) synthesis (By similarity). Involved in miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC . Modulates cell cycle progression and global translation of pre-B cells, its activation seems to be rate-limiting in tumorigenesis and tumor growth (By similarity).

Background References

1. Sanvito F. et.al. The beta4 integrin interactor p27(BBP/eIF6) is an essential nuclear matrix protein involved in 60S ribosomal subunit assembly. J. Cell Biol. 144:823-837(1999).

Subcellular Location

Centromere, Chromosome, Nucleus.

UNIPROT

SwissProt: P56537 Human

SwissProt: O55135 Mouse

Synonyms

b(2)gcn antibody

B(2)GCN homolog antibody

B4 integrin interactor antibody

Binding protein of beta-4 integrin antibody

CAB antibody

eIF-6 antibody

EIF3A antibody

EIF6 antibody

Eukaryotic translation initiation factor 3A antibody

Eukaryotic translation initiation factor 6 antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-54" style="font-weight: bold;text-decoration: underline;">ER2001-54</a>, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-54, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-54" style="font-weight: bold;text-decoration: underline;">ER2001-54</a>, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-eIF-6 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-54, 1/2,000) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of eIF-6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-54" style="font-weight: bold;text-decoration: underline;">ER2001-54</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of eIF-6 was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-54, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of eIF-6 on different lysates with Rabbit anti-eIF-6 antibody (<a href="/products/ER2001-54" style="font-weight: bold;text-decoration: underline;">ER2001-54</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: 293T cell lysate <br />Lane 3: K-562 cell lysate <br />Lane 4: C2C12 cell lysate<br />Lane 5: Mouse liver tissue lysate <br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Exposure time: 10 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER2001-54" style="font-weight: bold;text-decoration: underline;">ER2001-54</a>, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27 kDa

    Western blot analysis of eIF-6 on different lysates with Rabbit anti-eIF-6 antibody (ER2001-54) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: C2C12 cell lysate
    Lane 5: Mouse liver tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Exposure time: 10 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER2001-54, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

metafield image

eIF-6 Mouse Monoclonal Antibody [A6A2]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

eIF-6 Recombinant Mouse Monoclonal Antibody [A6A2-R]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

eIF-6 Mouse Monoclonal Antibody [A6A3]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

eIF-6 Recombinant Mouse Monoclonal Antibody [A6A3-R]

Application: WB,IHC-P,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated