Phospho-P38 (T180+Y182) Rabbit Polyclonal Antibody (ER2001-52)
Catalog# ER2001-52
Rabbit Polyclonal to Phospho-P38 (T180+Y182)
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WB
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Human
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Mouse
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Rat
Lane 1: NIH/3T3 cell lysate
Lane 2: NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute 29 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-52) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Phospho-P38 (T180+Y182) Rabbit Polyclonal Antibody (ER2001-52)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding thr180 and y182 of human p38.
Host
Rabbit
Modification
Phospho
Modification Site
T180+Y182
Positive Control
NIH/3T3 treated with 250ng/mL anisomycin for 30 minutes cell lysate, HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate, PC-12 treated with UV for 1 hour cell lysate.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-P38 (T180+Y182)
SYNONYMS
CSAID Binding Protein 1 antibody;CSAID binding protein antibody;CSAID-binding protein antibody;Csaids binding protein antibody;CSBP 1 antibody;CSBP 2 antibody;CSBP antibody;CSBP1 antibody;CSBP2 antibody;CSPB1 antibody;Cytokine suppressive anti-inflammatory drug-binding protein antibody;EXIP antibody;MAP kinase 14 antibody;MAP kinase MXI2 antibody;MAP kinase p38 alpha antibody;MAPK 14 antibody;MAPK14 antibody;MAX interacting protein 2 antibody;MAX-interacting protein 2 antibody;Mitogen Activated Protein Kinase 14 antibody;Mitogen activated protein kinase p38 alpha antibody;Mitogen-activated protein kinase 14 antibody;Mitogen-activated protein kinase p38 alpha antibody;MK14_HUMAN antibody;Mxi 2 antibody;MXI2 antibody;p38 ALPHA antibody;p38 antibody;p38 MAP kinase antibody;p38 MAPK antibody;p38 mitogen activated protein kinase antibody;p38ALPHA antibody;p38alpha Exip antibody;PRKM14 antibody;PRKM15 antibody;RK antibody;SAPK2A antibody;Stress-activated protein kinase 2a antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets.
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