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P2RX5 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-36

P2RX5 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Rat

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

P2RX5 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within rat P2RX5 aa 400-455.

Species Reactivity

Rat, Mouse

Validated Applications

WB, IHC-P

Target Molecular Weight

51 kDa

Positive Control

Rat brain tissue lysate, rat heart tissue lysate, mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

RRID

AB_3069514

Product Features

Form

Liquid

Concentration

1 mg/ml.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

Target

Function

P2X purinoceptor 5 is a protein that in humans is encoded by the P2RX5 gene. The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Several characteristic motifs of ATP-gated channels are present in its primary structure, but, unlike other members of the purinoceptors family, this receptor has only a single transmembrane domain. Four transcript variants encoding distinct isoforms have been identified for this gene.

Background References

1. Greig AV. et. al. Purinergic receptors are part of a functional signaling system for proliferation and differentiation of human epidermal keratinocytes. J. Invest. Dermatol. 120 : 1007–15(2003).

Subcellular Location

Membrane.

UNIPROT

SwissProt: P51578 Rat

SwissProt: Q3UYI1 Mouse

Synonyms

ATP receptor antibody

ATP receptor subunit antibody

Ionotropic ATP receptor P2X5 antibody

LRH 1 antibody

LRH1 antibody

Lymphoid restricted histocompatibility antigen 1 antibody

MGC47755 antibody

P2rx5 antibody

P2RX5_HUMAN antibody

P2X purinoceptor 5 antibody

Expand

Images

  • Western blot analysis of P2RX5 on 10% mouse heart lysate with Rabbit anti-P2RX5 antibody (<a href="/products/ER2001-36" style="font-weight: bold;text-decoration: underline;">ER2001-36</a>) at 1/1,000 dilution<br /><br />Exposure time: 60 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER2001-36" style="font-weight: bold;text-decoration: underline;">ER2001-36</a>, 1/1,000 in 5% BSA, 2 hours at room temperature<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1:5,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 51 kDa<br />Observed band size: 51 kDa

    Western blot analysis of P2RX5 on 10% mouse heart lysate with Rabbit anti-P2RX5 antibody (ER2001-36) at 1/1,000 dilution

    Exposure time: 60 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER2001-36, 1/1,000 in 5% BSA, 2 hours at room temperature
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1:5,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-36" style="font-weight: bold;text-decoration: underline;">ER2001-36</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-36" style="font-weight: bold;text-decoration: underline;">ER2001-36</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2RX5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-36, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"