IRS1 Rabbit Polyclonal Antibody (ER2001-35)
Catalog# ER2001-35
Rabbit Polyclonal to IRS1
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Rat
Specifications
Product Type
Rabbit polyclonal primary
Product Name
IRS1 Rabbit Polyclonal Antibody (ER2001-35)
Immunogen
Recombinant protein within human irs1 aa 1-300.
Host
Rabbit
Positive Control
Rat brain tissue lysates, A549, MCF-7, human colon tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:100
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IF-Cell
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
132 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
IRS1
SYNONYMS
HIRS 1 antibody;HIRS1 antibody;Insulin receptor substrate 1 antibody;IRS 1 antibody;IRS-1 antibody;IRS1 antibody;IRS1_HUMAN antibody;OTTHUMP00000164234 antibody
SUBCELLULAR LOCATION
Cytosol, Nucleus.
FUNCTION
Insulin receptor substrate 1 plays a key role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways PI3K / Akt and Erk MAP kinase pathways. Tyrosine phosphorylation of IRS-1 by insulin receptor (IR) introduces multiple binding sites for proteins bearing SH2 homology domain, such as PI3K, Grb-2/Sos complex and SHP2. PI3K, involved in interaction with IRS-1, produces PIP3, which, in turn, recruits Akt kinase. Further, Akt kinase is activated via phosphorylation of its T308 residue and analogous sites in PKC by PDK1. This phosphorylation is absent in tissues lacking IRS-1. The cascade is followed by glucose uptake. Formation of the Grb-2/Sos complex, also known as the RAS guanine nucleotide exchange factor complex, results in ERK1/2 activation. IRS-1 signal transduction may be inhibited by SHP2 in some tissues.
CITATIONS
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Li, J., Mao, Y. S., Chen, F., Xia, D. X., & Zhao, T. Q.
Palmitic acid up regulates Gal-3 and induces insulin resistance in macrophages by mediating the balance between KLF4 and NF-κB
