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Anti-Androgen Receptor Antibody

Usd: 315

Specification

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Catalog# ER2001-34

Anti-Androgen Receptor Antibody

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Anti-Androgen Receptor Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human Androgen Receptor aa 200-500.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, FC

Target Molecular Weight

99 kDa

Positive Control

Human skeletal muscle tissue lysate, rat heart tissue lysate, A549, MCF-7.

Conjugation

unconjugated

RRID

AB_3069512

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation . Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.9 Publications Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.

Background References

1. Sun C.et.al.Discovery of potent, orally-active, and muscle-selective androgen receptor modulators based on an N-aryl-hydroxybicyclohydantoin scaffold.J. Med. Chem. 49:7596-7599(2006).

Subcellular Location

Cytoplasm, Nucleus

UNIPROT

P10275

Synonyms

AIS antibody

ANDR_HUMAN antibody

Androgen nuclear receptor variant 2 antibody

Androgen receptor (dihydrotestosterone receptor

testicular feminization

spinal and bulbar muscular atrophy

Kennedy disease) antibody

Androgen receptor antibody

androgen receptor splice variant 4b antibody

AR antibody

Expand

Images

  • Western blot analysis of Androgen Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-34" style="font-weight: bold;text-decoration: underline;">ER2001-34</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br />Positive control: <br />Lane 1: Human skeletal muscle tissue lysate<br />Lane 2: Rat heart tissue lysate

    Western blot analysis of Androgen Receptor on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Human skeletal muscle tissue lysate
    Lane 2: Rat heart tissue lysate

  • ICC staining of Androgen Receptor in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/ER2001-34" style="font-weight: bold;text-decoration: underline;">ER2001-34</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

    ICC staining of Androgen Receptor in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER2001-34, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

  • Flow cytometric analysis of Androgen Receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-34" style="font-weight: bold;text-decoration: underline;">ER2001-34</a>, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Androgen Receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-34, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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Conjugate: unconjugated