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PYCR1 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-18

PYCR1 Rabbit Polyclonal Antibody

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PYCR1 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human PYCR1 aa 220-260.

Species Reactivity

Human

Validated Applications

WB, FC

Target Molecular Weight

Predicted band size: 33 kDa

Positive Control

K-562 cell lysate, 293T cell lysate, A549 cell lysate, HepG2.

Conjugation

unconjugated

RRID

AB_3069497

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

Target

Function

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants.

Background References

1. Milne K. et. al. A fragment-like approach to PYCR1 inhibition. Bioorg Med Chem Lett. 2019 Sep

2. Weijin F. et. al. The clinical significance of PYCR1 expression in renal cell carcinoma. Medicine (Baltimore). 2019 Jul

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: P32322 Human

Synonyms

P5C antibody

P5C reductase 1 antibody

P5C reductase antibody

P5CR 1 antibody

P5CR antibody

PIG45 antibody

PP222 antibody

Proliferation inducing protein 45 antibody

PYCR 1 antibody

PYCR antibody

Expand

Images

  • Western blot analysis of PYCR1 on different lysates with Rabbit anti-PYCR1 antibody (<a href="/products/ER2001-18" style="font-weight: bold;text-decoration: underline;">ER2001-18</a>) at 1/1,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: 293T cell lysate<br />Lane 3: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 33 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 25 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-18" style="font-weight: bold;text-decoration: underline;">ER2001-18</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PYCR1 on different lysates with Rabbit anti-PYCR1 antibody (ER2001-18) at 1/1,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: 293T cell lysate
    Lane 3: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 33 kDa
    Observed band size: 33 kDa

    Exposure time: 25 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-18) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of PYCR1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-18" style="font-weight: bold;text-decoration: underline;">ER2001-18</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PYCR1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-18, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"