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SAP97 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER2001-07

SAP97 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

SAP97 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human aa 1-100.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 100 kDa

Positive Control

Siha cell lysate, A431 cell lysate, human liver carcinoma tissue, mouse colon tissue, A431.

Conjugation

unconjugated

RRID

AB_3069487

Product Features

Form

Liquid

Concentration

1mg/mL(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.This gene encodes a multi-domain scaffolding protein that is required for normal development. This protein may have a role in septate junction formation, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene, but the full-length nature of some of the variants is not known.

Background References

1. El-Haou S. et. al. Kv4 potassium channels form a tripartite complex with the anchoring protein SAP97 and CaMKII in cardiac myocytes. Circ. Res. 104:758-769(2009)

Subcellular Location

Cell junction, Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Synapse.

UNIPROT

SwissProt: Q12959 Human

SwissProt: Q811D0 Mouse

SwissProt:Q62696 Rat

Synonyms

Discs large homolog 1 antibody

discs large, Drosophila, homolog of, 1 antibody

discs, large homolog 1 (Drosophila) antibody

Disks large homolog 1 antibody

dJ1061C18.1.1 antibody

DKFZp761P0818 antibody

DKFZp781B0426 antibody

DLG 1 antibody

DLG1 antibody

DLG1_HUMAN antibody

Expand

Images

  • Western blot analysis of SAP97 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER2001-07" style="font-weight: bold;text-decoration: underline;">ER2001-07</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.<br /><span style="font-weight: bold;">Positive control:</span> <br />Lane 1: Siha cell lysate<br />Lane 2: A431 cell lysate

    Western blot analysis of SAP97 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-07, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
    Positive control:
    Lane 1: Siha cell lysate
    Lane 2: A431 cell lysate

  • Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-07" style="font-weight: bold;text-decoration: underline;">ER2001-07</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-07, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-07" style="font-weight: bold;text-decoration: underline;">ER2001-07</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SAP97 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-07, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of SAP97 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-07" style="font-weight: bold;text-decoration: underline;">ER2001-07</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of SAP97 was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-07, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Alternative Products

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SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

Products with the same target and pathway

metafield image

SAP97 Recombinant Rabbit Monoclonal Antibody [JE39-74]

Application: WB,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated