Skip to content

CD180 Rabbit Polyclonal Antibody

Usd: 315

Specification

Bulk Order Quote | Customization Request

Catalog# ER2001-06

CD180 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CD180 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within human CD180 aa 370-580.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Target Molecular Weight

Predicted band size: 74 kDa

Positive Control

Raji (Human Burkitt's lymphoma cell) cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, rat spleen tissue, human spleen tissue, mouse spleen tissue, Daudi.

Conjugation

unconjugated

RRID

AB_3073453

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:10,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

CD180 antigen is a protein that in humans is encoded by the CD180 gene. CD180 is a cell surface molecule consisting of extracellular leucine-rich repeats (LRR) and a short cytoplasmic tail. It is also known by the archaic terms Bgp-95 and RP105, for the founding designations following discovery in humans (1988) and mice (1994), respectively. CD180 is expressed on antigen presenting cells including B cells and dendritic cells. The extracellular LRR is associated with a molecule called MD-1 and form the cell surface receptor complex, CD180/MD-1. It belongs to the family of pathogen receptors, Toll-like receptors (TLR). CD180/MD-1, by working in concert with TLR4, controls B cell recognition and signaling of lipopolysaccharide (LPS), a membrane constituent of Gram-negative bacteria. Recently, CD180 has been demonstrated to be involved in the survival and prognosis of B-cell chronic lymphocytic leukemia.

Background References

1. Yang Y. et. al. CD180 Ligation Inhibits TLR7- and TLR9-Mediated Activation of Macrophages and Dendritic Cells Through the Lyn-SHP-1/2 Axis in Murine Lupus. Front Immunol. 2018 Nov

2. Kikuchi J. et. al. Myeloma Cells Are Activated in Bone Marrow Microenvironment by the CD180/MD-1 Complex, Which Senses Lipopolysaccharide. Cancer Res. 2018 Apr

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q99467 Human

SwissProt: Q62192 Mouse

Synonyms

CD180 antibody

CD180 antigen antibody

CD180_HUMAN antibody

F630107B15 antibody

LY64 antibody

Ly78 antibody

Lymphocyte antigen 64 antibody

lymphocyte antigen 64 radioprotective 105kDa antibody

lymphocyte antigen-64, radioprotective, 105kDa antibody

MGC126233 antibody

Expand

Images

  • Western blot analysis of CD180 on different lysates with Rabbit anti-CD180 antibody (<a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>) at 1/10,000 dilution.<br /><br />Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate<br />Lane 2: Mouse spleen tissue lysate<br />Lane 3: Rat spleen tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 25 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 74 kDa<br />Observed band size: 90 kDa

    Western blot analysis of CD180 on different lysates with Rabbit anti-CD180 antibody (ER2001-06) at 1/10,000 dilution.

    Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
    Lane 2: Mouse spleen tissue lysate
    Lane 3: Rat spleen tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 25 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: ER2001-06, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 74 kDa
    Observed band size: 90 kDa

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-06, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-06, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD180 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER2001-06, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of CD180 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (<a href="/products/ER2001-06" style="font-weight: bold;text-decoration: underline;">ER2001-06</a>, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of CD180 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (ER2001-06, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"