TRPM4 Rabbit Polyclonal Antibody
Catalog# ER1902-81
TRPM4 Rabbit Polyclonal Antibody
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WB
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TRPM4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human TRPM4 aa 1-50 / 1,214.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, FC
Target Molecular Weight
Predicted band size 134 kDa
Positive Control
Mouse colon tissue lysates, MCF-7.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:1,000
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FC
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1:50-1:100
Target
Function
The protein encoded by this gene is a calcium-activated nonselective ion channel that mediates transport of monovalent cations across membranes, thereby depolarizing the membrane. The activity of the encoded protein increases with increasing intracellular calcium concentration, but this channel does not transport calcium.
Background References
1. Gao Y. et. al. TRPM4 channel and cancer. Cancer Lett. 2019 Jul 10;454:66-69.
2. Autzen HE. et. al. Structure of the human TRPM4 ion channel in a lipid nanodisc. Science. 2018 Jan 12;359(6372):228-232.
Sequence Similarity
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM4 sub-subfamily.
Tissue Specificity
Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells. Expressed in keratocytes.
Post-translational Modification
Phosphorylation by PKC leads to increase the sensitivity to Ca(2+).; Sumoylated. Desumoylated by SENP1.
Subcellular Location
Cell membrane, endoplasmic reticulum, golgi apparatus.
Synonyms
1110030C19Rik antibody
AW047689 antibody
Calcium-activated non-selective cation channel 1 antibody
FLJ20041 antibody
hTRPM4 antibody
Long transient receptor potential channel 4 antibody
LTrpC-4 antibody
LTrpC4 antibody
Melastatin 4 antibody
Melastatin like 2 protein antibody
ExpandImages
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Western blot analysis of TRPM4 on mouse colon tissue lysates with Rabbit anti-TRPM4 antibody (ER1902-81) at 1/1,000 dilution.
Lysates/proteins at 40 µg/Lane.
Predicted band size: 134 kDa
Observed band size: 134 kDa
Exposure time: 1 minute 7 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Flow cytometric analysis of TRPM4 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-81, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"