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RAB11FIP3 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-80

RAB11FIP3 Rabbit Polyclonal Antibody

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

RAB11FIP3 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within mouse RAB11FIP3 aa 543-592 / 1,047.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 119 kDa

Positive Control

U-87 MG cell lysate, AGS cell lysate, Mouse liver tissue lysate, Mouse brain tissue lysate, Rat liver tissue lysate, Rat brain tissue lysate, human liver tissue, human kidney tissue.

Conjugation

unconjugated

RRID

AB_3069463

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:5,000

Target

Function

Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track).

Background References

1. Tong D. et. al. Increased Eps15 homology domain 1 and RAB11FIP3 expression regulate breast cancer progression via promoting epithelial growth factor receptor recycling. Tumour Biol. 2017 Feb

2. Bouchet J. et. al. Rab11-FIP3 Regulation of Lck Endosomal Traffic Controls TCR Signal Transduction. J Immunol. 2017 Apr

Post-translational Modification

Phosphorylated at Ser-102 by CDK1 during metaphase, and dephosphorylated as cells enter telophase.

Subcellular Location

Endosome membrane, Recycling endosome membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cleavage furrow, Midbody, Golgi apparatus membrane, Golgi apparatus, trans-Golgi network membrane.

UNIPROT

SwissProt: O75154 Human

SwissProt: Q8CHD8 Mouse

Entrez Gene: 303002 Rat

Synonyms

EF hands containing Rab interacting protein antibody

Eferin antibody

Rab11 family interacting protein 3 antibody

RAB11FIP3 antibody

RFIP3 antibody

Images

  • Western blot analysis of RAB11FIP3 on different lysates with Rabbit anti-RAB11FIP3 antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution.<br /><br />Lane 1: U-87 MG cell lysate<br />Lane 2: AGS cell lysate<br />Lane 3: Mouse liver tissue lysate<br />Lane 4: Mouse brain tissue lysate<br />Lane 5: Rat liver tissue lysate<br />Lane 6: Rat brain tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 119 kDa<br />Observed band size: 119 kDa<br /><br />Exposure time: 18 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of RAB11FIP3 on different lysates with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution.

    Lane 1: U-87 MG cell lysate
    Lane 2: AGS cell lysate
    Lane 3: Mouse liver tissue lysate
    Lane 4: Mouse brain tissue lysate
    Lane 5: Rat liver tissue lysate
    Lane 6: Rat brain tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 119 kDa
    Observed band size: 119 kDa

    Exposure time: 18 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-80) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RAB11FIP3 antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RAB11FIP3 antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ER1902-80" style="font-weight: bold;text-decoration: underline;">ER1902-80</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-RAB11FIP3 antibody (ER1902-80) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-80) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"