SynGAP Rabbit Polyclonal Antibody
Catalog# ER1902-57
SynGAP Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SynGAP Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within human SynGAP aa 1100-1250.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
148 kDa
Positive Control
Mouse brain tissue lysate, human terus tissue, mouse brain tissue, SW620.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:100
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FC
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1:50-1:100
Target
Function
Synaptic Ras GTPase-activating protein 1, also known as synaptic Ras-GAP 1 or SYNGAP1, is a protein that in humans is encoded by the SYNGAP1 gene. SYNGAP1 is a ras GTPase-activating protein that is critical for the development of cognition and proper synapse function. Mutations in humans can cause intellectual disability or epilepsy. SynGAP1 is a complex protein with several functions that may be regulated temporally via complex isoforms. A well-documented function of SynGAP1 involves NMDA receptor-mediated synaptic plasticity and membrane insertion of AMPA receptors through the suppression of upstream signaling pathways. However, SynGAP1 has also been shown to function cooperatively with Unc51.1 in axon formation. One way SynGAP1 affects these processes is through the MAP kinase signaling pathway by attenuation of Ras signalling. However, alternative splicing and multiple translational start sites have been shown to cause opposing effects, illustrating the importance of multiple functional domains that reside within the c- and n-termini. For example, the expression of an α1 or α2 c-terminal variant of SynGAP1 will either increase or decrease synaptic strength, respectively. Overall, SynGAP1 is essential for development and survival, which is evident as knockout mice die perinatally.
Background References
1. Hamdan F.F.et.al.Mutations in SYNGAP1 in autosomal nonsyndromic mental retardation.N. Engl. J. Med. 360:599-605(2009).
Post-translational Modification
Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Phosphorylation by PLK2 promotes its activity (By similarity).
Subcellular Location
Cytosol.
Synonyms
DKFZp761G1421 antibody
KIAA1938 antibody
MRD5 antibody
Neuronal RasGAP antibody
OTTHUMP00000064825 antibody
p135 SynGAP antibody
Ras GTPase activating protein SynGAP antibody
Ras GTPase-activating protein SynGAP antibody
RASA 1 antibody
RASA 5 antibody
ExpandImages
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Western blot analysis of SynGAP on Mouse brain tissue lysate with Rabbit anti-SynGAP antibody (ER2001-04) at 1/500 dilution.
Lysates/proteins at 40 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER2001-04, 1/500 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 148 kDa
Observed band size: 148 kDa -
Immunohistochemical analysis of paraffin-embedded human terus tissue using anti-SynGAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-57, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SynGAP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-57, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of SynGAP was done on SW620 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-57, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
SynGAP Recombinant Rabbit Monoclonal Antibody [JE57-79]
Application: WB,IHC-P,IF-Tissue
Reactivity: Mouse,Rat
Conjugate: unconjugated
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