LGR5 Rabbit Polyclonal Antibody
Catalog# ER1902-52
LGR5 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
LGR5 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human LGR5 aa 499-548 / 907.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 100 kDa
Positive Control
HEK-293 (Human embryonic kidney cell) cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.
Background References
1. Kantara C et al. Methods for detecting circulating cancer stem cells (CCSCs) as a novel approach for diagnosis of colon cancer relapse/metastasis. Lab Invest 95:100-12 (2015).
2. Oshima N et al. Induction of cancer stem cell properties in colon cancer cells by defined factors. PLoS One 9:e101735 (2014).
Sequence Similarity
Belongs to the G-protein coupled receptor 1 family.
Tissue Specificity
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
Subcellular Location
Cell membrane, Golgi apparatus.
UNIPROT
Synonyms
FEX antibody
G protein coupled receptor 49 antibody
G protein coupled receptor 67 antibody
g protein-coupled receptor fex antibody
G-protein coupled receptor 49 antibody
G-protein coupled receptor 67 antibody
G-protein coupled receptor HG38 antibody
GPCR GPR49 antibody
GPR 49 antibody
GPR 67 antibody
ExpandImages
-
Western blot analysis of LGR5 on HEK-293 (Human embryonic kidney cell) cell lysate with Rabbit anti-LGR5 antibody (ER1902-52) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1902-52, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
