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Flavin-containing monooxygenase Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-45

Flavin-containing monooxygenase Rabbit Polyclonal Antibody

Application

  • WB

Reactivity

  • Rice

Conjugation

  • unconjugated

Overview

Product Name

Flavin-containing monooxygenase Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within rice Flavin-containing monooxygenase aa 370-419 / 419.

Species Reactivity

Rice

Validated Applications

WB

Molecular Weight

46 kDa

Positive Control

Rice tissue lysates.

Conjugation

unconjugated

RRID

AB_3069429

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

FMOs are one subfamily of class B external flavoprotein monooxygenases (EC 1.14.13), which belong to the family of monooxygenase oxidoreductases, along with the other subfamilies Baeyer-Villiger monooxygenases and microbial N-hydroxylating monooxygenases. FMO's are found in fungi, yeast, plants, mammals, and bacteria. Plant FMO's play a role in defending against pathogens and catalyze specific steps in the biosynthesis of auxin, a plant hormone. Plant FMO's also play a role in the metabolism of glucosinolates. These non-xenobiotic roles of plant FMO's suggest that other FMO functions could be identified in non-plant organisms.

Background References

1. Schlaich NL (September 2007). Flavin-containing monooxygenases in plants: looking beyond detox. Trends in Plant Science. 12 (9): 412–418.

Sequence Similarity

Belongs to the FMO family.

Subcellular Location

Integral component of membrane.

UNIPROT

SwissProt: A2XPX7 Rice

Synonyms

Flavin-containing monooxygenase

OsI_14691

Images

  • Western blot analysis of Flavin-containing monooxygenase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER1902-45" style="font-weight: bold;text-decoration: underline;">ER1902-45</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Flavin-containing monooxygenase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-45, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"