Flavin-containing monooxygenase Rabbit Polyclonal Antibody
Catalog# ER1902-44
Flavin-containing monooxygenase Rabbit Polyclonal Antibody
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WB
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IF-Tissue
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IHC-P
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Rice
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unconjugated
Overview
Product Name
Flavin-containing monooxygenase Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within rice Flavin-containing monooxygenase aa 1-50 / 419.
Species Reactivity
Rice
Validated Applications
WB, IF-Tissue, IHC-P
Target Molecular Weight
46 kDa
Positive Control
Rice tissue lysates, rice tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:2,000
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IF-Tissue
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1:50-1:100
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IHC-P
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1:50-1:200
Target
Function
FMOs are one subfamily of class B external flavoprotein monooxygenases (EC 1.14.13), which belong to the family of monooxygenase oxidoreductases, along with the other subfamilies Baeyer-Villiger monooxygenases and microbial N-hydroxylating monooxygenases. FMO's are found in fungi, yeast, plants, mammals, and bacteria. Plant FMO's play a role in defending against pathogens and catalyze specific steps in the biosynthesis of auxin, a plant hormone. Plant FMO's also play a role in the metabolism of glucosinolates. These non-xenobiotic roles of plant FMO's suggest that other FMO functions could be identified in non-plant organisms.
Background References
1. Schlaich NL (September 2007). Flavin-containing monooxygenases in plants: looking beyond detox. Trends in Plant Science. 12 (9): 412–418.
Sequence Similarity
Belongs to the FMO family.
Subcellular Location
Integral component of membrane.
UNIPROT
Synonyms
Flavin-containing monooxygenase
OsI_14691
Images
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Western blot analysis of Flavin-containing monooxygenase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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Immunofluorescence staining of paraffin-embedded rice tissue using anti-Flavin-containing monooxygenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-44 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Flavin-containing monooxygenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"