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SARS2 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER1902-38

SARS2 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Tissue

  • IHC-P

Reactivity

  • Zebrafish

Conjugation

  • unconjugated

Overview

Product Name

SARS2 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Recombinant protein within zebrafish SARS2 aa 289-510 / 510.

Species Reactivity

Zebrafish

Validated Applications

WB, IF-Tissue, IHC-P

Molecular Weight

Predicted band size: 58 kDa

Positive Control

Zebrafish tissue lysate, Zebrafish tissue.

Conjugation

unconjugated

RRID

AB_3069422

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Tissue

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

Target

Function

Involved in ATP binding, has serine-tRNA ligase activity and involved in seryl-tRNA aminoacylation. The 3 substrates of this enzyme are ATP, L-serine, and tRNA(Ser), whereas its 3 products are AMP, diphosphate, and L-seryl-tRNA(Ser). This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-serine:tRNASer ligase (AMP-forming). Other names in common use include seryl-tRNA synthetase, SerRS, seryl-transfer ribonucleate synthetase, seryl-transfer RNA synthetase, seryl-transfer ribonucleic acid synthetase, and serine translase. This enzyme participates in glycine, serine and threonine metabolism and aminoacyl-trna biosynthesis.

Background References

1. Gaudet P et al. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12:449-462 (2011).

Subcellular Location

Mitochondrion.

UNIPROT

SwissProt: A3QJX4 Zebrafish

Synonyms

mitochondrial antibody

mtSerRS antibody

SARS antibody

SARS2 antibody

SARSM antibody

Serine tRNA ligase antibody

Serine--tRNA ligase antibody

SerRS antibody

SerRSmt antibody

SERS antibody

Expand

Images

  • Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (<a href="/products/ER1902-38" style="font-weight: bold;text-decoration: underline;">ER1902-38</a>, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  • IF staining of SARS2 in Zebrafish tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    IF staining of SARS2 in Zebrafish tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"