SARS2 Rabbit Polyclonal Antibody
Catalog# ER1902-38
SARS2 Rabbit Polyclonal Antibody
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WB
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IF-Tissue
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IHC-P
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Zebrafish
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unconjugated
Overview
Product Name
SARS2 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Recombinant protein within zebrafish SARS2 aa 289-510 / 510.
Species Reactivity
Zebrafish
Validated Applications
WB, IF-Tissue, IHC-P
Target Molecular Weight
Predicted band size: 58 kDa
Positive Control
Zebrafish tissue lysate, Zebrafish tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
Target
Function
Involved in ATP binding, has serine-tRNA ligase activity and involved in seryl-tRNA aminoacylation. The 3 substrates of this enzyme are ATP, L-serine, and tRNA(Ser), whereas its 3 products are AMP, diphosphate, and L-seryl-tRNA(Ser). This enzyme belongs to the family of ligases, to be specific those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. The systematic name of this enzyme class is L-serine:tRNASer ligase (AMP-forming). Other names in common use include seryl-tRNA synthetase, SerRS, seryl-transfer ribonucleate synthetase, seryl-transfer RNA synthetase, seryl-transfer ribonucleic acid synthetase, and serine translase. This enzyme participates in glycine, serine and threonine metabolism and aminoacyl-trna biosynthesis.
Background References
1. Gaudet P et al. Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12:449-462 (2011).
Subcellular Location
Mitochondrion.
UNIPROT
Synonyms
mitochondrial antibody
mtSerRS antibody
SARS antibody
SARS2 antibody
SARSM antibody
Serine tRNA ligase antibody
Serine--tRNA ligase antibody
SerRS antibody
SerRSmt antibody
SERS antibody
ExpandImages
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Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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IF staining of SARS2 in Zebrafish tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"