TMX4 Rabbit Polyclonal Antibody
Catalog# ER1902-31
TMX4 Rabbit Polyclonal Antibody
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WB
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
TMX4 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human TMX4 aa 300-349 / 349.
Species Reactivity
Human
Validated Applications
WB, IHC-P, FC
Target Molecular Weight
Predicted band size: 39 kDa
Positive Control
Daudi cell lysate, human skin tissue lysate, human tonsil tissue, human liver carcinoma tissue, human colon carcinoma tissue, human breast carcinoma tissue, SH-SY5Y.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
Target
Function
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, one transmembrane domain and C-terminal ASP/GLU-rich calcium binding domain. Unlike most members of this gene family, it lacks a C-terminal ER-retention sequence. The encoded protein has been shown to have reductase activity in vitro.
Background References
1. Sugiura Y. et. al. Novel thioredoxin-related transmembrane protein TMX4 has reductase activity. J Biol Chem. 2010 Mar 5;285(10):7135-42.
2. Roth D. et. al. A di-arginine motif contributes to the ER localization of the type I transmembrane ER oxidoreductase TMX4. Biochem J. 2009 Dec 14;425(1):195-205.
Subcellular Location
Nucleus inner membrane.
UNIPROT
Synonyms
DJ971N18.2 antibody
KIAA1162 antibody
PDIA14 antibody
Protein disulfide isomerase family A 14 antibody
PSEC0095 antibody
Thioredoxin domain containing 13 antibody
Thioredoxin domain containing protein 13 antibody
Thioredoxin related transmembrane protein 4 antibody
TMX4 antibody
TXNDC 13 antibody
ExpandImages
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Western blot analysis of TMX4 on different lysates with Rabbit anti-TMX4 antibody (ER1902-31) at 1/1,000 dilution.
Lane 1: 293T (Human embryonic kidney cell) cell lysate
Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 3: A375 (Human amelanotic melanoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1902-31, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 39 kDa
Observed band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TMX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-TMX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-TMX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-TMX4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-31, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of TMX4 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-31, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"